Abstract
Fatty acid desaturase (FAD) plays a crucial role in plant growth, development, and stress response. The FADs have been identified and reported in diverse plants, but so far, the whole genome exploration and analysis of FADs in castor (Ricinus communis L.) have not been reported yet. In this study, the identification of the RcFADs was carried out using a sequence alignment method. The physicochemical properties, gene structure, protein motifs, evolution, as well as gene expression under cold stress and different tissues were analyzed. A total of 16 RcFADs in the castor genome were identified, which contained the TMEM189_B_domain (PF10520)/FA_desaturase (PF00487)/FA_desaturase_2 (PF03405) conserved domain. Phylogenetic analysis showed that RcFADs can be categorized into ω-3, ω-6, ADS/SLD/DES, FAD4, and FAB2 groups, indicating that the function of RcFADs may vary. RNA-Sequencing and real-time PCR showed that the expression of RcFAD4 and RcFAD8.1 were increased with the duration of cold treatments, indicating their involvement in cold adaptability of castor. Global expression profiles of the RcFADs in different tissues displayed diverse expression patterns, which suggested that these genes might play important roles in regulating different developmental and physiological processes in castor. This study provides valuable information for understanding the potential function of RcFADs in regulating the growth and development and abiotic stress responses in castor.
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The data supporting the findings of this study are available upon reasonable request from the corresponding author.
Abbreviations
- FAD:
-
Fatty acid desaturase
- FAs:
-
Fatty acids
- pI:
-
Isoelectric points
- MW:
-
Molecular weight
- JA:
-
Jasmonic acid
- PSI:
-
Photosystem I
- PSII:
-
Photosystem II
- ROS:
-
Reactive oxygen species
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Funding
The National Natural Science Foundation of China (32060493;32360463;31760399). The 22nd Batch of High-level Talent Development Funds of Inner Mongolia Autonomous Region. Entrepreneurship Startup Project for Overseas Scholar (Grant No. 2020QNCXRC07). Basic Research Project of Inner Mongolia Autonomous Region Directly administered Universities (GXKY22056). The Program for Young Talents of Science and Technology in the University of Inner Mongolia Autonomous Region (Grant No. NJYT-20-B25). The Doctoral Program Fund of Inner Mongolia Minzu University (Grant No. BS398).
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Xiaoyu Wang and Ying Wu conceived and designed the experiments. Mengdi Sun and Jixing Zhang performed the experiments. Mengdi Sun, Jixing Zhang, Nan Wang, Xue Wei, Hong Fang, Xue Ding, Hui Xu, Xiumin Yu, Hongyan Huo, Ying Wu, and Xiaoyu Wang analyzed the data. Mengsi Sun and Xiaoyu Wang wrote the manuscript. All authors reviewed and edited the manuscript.
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Sun, M., Zhang, J., Wang, N. et al. Genome-Wide Identification, Phylogenetic and Expression Pattern Analysis of Fatty Acid Desaturase Genes in Castor (Ricinus communis L.). Tropical Plant Biol. 17, 52–64 (2024). https://doi.org/10.1007/s12042-023-09349-7
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DOI: https://doi.org/10.1007/s12042-023-09349-7