Abstract
Elicitation in a simple and efficient strategy to increase plant functional metabolites. This work aimed to determine the effect of salicylic acid foliar application on biomass production and the synthesis of bioactive compounds in red beetroot (Beta vulgaris L.). The experiment was conducted on potted plants under greenhouse conditions. The application of salicylic acid was carried out at concentrations of 0.0, 0.5, 1.0, 1.5 and 2.0 mmol L−1 for three consecutive days in the vegetative phase. Plants treated with 0.5 and 2.0 mmol L−1 salicylic acid showed higher fresh and dry weight values (leaf, root and total), leaf area (total and specific), number of leaves, leaf mass ratio, root diameter and length and productivity. Salicylic acid application of 1.5 mmol L−1 increases chlorophyll, total soluble solids and flavonoids concentrations. The elicitor effect was observed at 1.0 mmol L−1 of salicylic acid, resulting in greater economic value of the biomass in the higher concentrations of polyphenols, anthocyanins, β-carotene, lutein, betacyanin, betaxanthin and total betalains. Additionally, an increase in the root antioxidant activity was obtained at the concentrations of 1.0 and 2.0 mmol L−1. The elicitation of red beetroot by salicylic acid foliar pulverisation during the vegetative growth is efficient to promote higher productivity of roots and nutritional value.
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References
Akbudak N, Zambi O, Duran UT (2022) Evaluation of Exogenous Salicylic Acid Application on White Mould Disease (Sclerotinia sclerotiorum) and Photosynthetic Pigments in Lettuce (Lactuca sativa L.). Turk J Agric Res 9:90–96. https://doi.org/10.19159/tutad.1056333
Ali B (2021) Salicylic acid An efficient elicitor of secondary metabolite production in plants. Biocatal Agric Biotechnol 31:101884. https://doi.org/10.1016/j.bcab.2020.101884
Aminifard MH, Jorkesh A, Fallahi HR, Moslemi FS (2020) Influences of benzyl adenine and salicylic acid and on growth, yield, and biochemical characteristics of coriander (Coriandrum sativum L.). S Afr J Bot 132:299–303. https://doi.org/10.1016/j.sajb.2020.05.019
Arif Y, Sami F, Siddiqui H, Bajguz A, Hayat S (2020) Salicylic acid in relation to other phytohormones in plant: A study towards physiology and signal transduction under challenging environment. Environ Exp Bot 175:104040. https://doi.org/10.1016/j.envexpbot.2020.104040
Blois MS (1958) Antioxidant determinations by the use of a stable free radical. Nature 181:1199. https://doi.org/10.1038/1811199a0
Chavoushi M, Najafi F, Salimi A, Angaji SA (2020) Effect of salicylic acid and sodium nitroprusside on growth parameters, photosynthetic pigments and secondary metabolites of safflower under drought stress. Sci Hortic 259:108823. https://doi.org/10.1016/j.scienta.2019.108823
Chhikara N, Kushwaha K, Sharma P, Gat Y, Panghal A (2019) Bioactive compounds of beetroot and utilization in food processing industry: A critical review. Food Chem 272:192–200. https://doi.org/10.1016/j.foodchem.2018.08.022
Craft NE, Soares JH Jr (1992) Relative solubility, stability and absorptivity of lutein and beta-carotene in organic solvents. J Agric Food Chem 40:431–434. https://doi.org/10.1021/jf00015a013
Einhardt PM, Lima CSM, de Andrade SB (2017) Salicylic acid in the post-harvest conservation of fruits of Physalis peruviana L. Rev Iber Tecnol Postcosecha 18:53–59
El Sherif F, Alkuwayti MA, Khattab S (2022) Foliar Spraying of Salicylic Acid Enhances Growth, Yield, and Curcuminoid Biosynthesis Gene Expression as Well as Curcuminoid Accumulation in Curcuma longa. Hortic 8:417. https://doi.org/10.3390/horticulturae8050417
Fernandez-Panchon MS, Villano D, Troncoso AM, Garcia-Parrilla MC (2008) Antioxidant activity of phenolic compounds: From in vitro results to in vivo evidence. Crit Rev Food Sci Nutr 48:649–671. https://doi.org/10.1080/10408390701761845
Ferreira DF (2019) SISVAR: a computer analysis system to fixed effects split plot type designs. Braz J Biomet 37:529–535. https://doi.org/10.28951/rbb.v37i4.450
Francis FJ (1982) Analysis of anthocyanins. In: Markakis P (ed) Anthocyanins as Food Colors. Academic Press, New York, pp 182–205
Fu Y, Shi J, Xie SY, Zhang TY, Soladoye OP, Aluko RE (2020) Red beetroot betalains: Perspectives on extraction, processing, and potential health benefits. J Agric Food Chem 68:11595–11611. https://doi.org/10.1021/acs.jafc.0c04241
Gonçalves FCDM, Parreiras NDS, Campos FG, Mantoan LPB, Boaro CSF (2020) Exogenous salicylic acid modifies gas exchange and biomass production of Mentha x piperita L. Aust J Crop Sci 14:98–107. https://doi.org/10.21475/ajcs
Gondor OK, Janda T, Soós V, Pál M, Majláth I, Adak MK, Balázs E, Szalai G (2016) Salicylic acid induction of flavonoid biosynthesis pathways in wheat varies by treatment. Front Plant Sci 7:1447. https://doi.org/10.3389/fpls.2016.01447
Gorni PH, Brozulato MDO, Lourenção RDS, Konrad ECG (2017) Increased biomass and salicylic acid elicitor activity in fennel (Foeniculum vulgare Miller). Braz J Food Technol 20:e2016172. https://doi.org/10.1590/1981-6723.17216
Gorni PH, Cornelissen BS, Pereira AA (2021) Exogenous salicylic acid and ferulic acid improve growth, phenolic and carotenoid content in tomato. Adv Hortic Sci 35:335–341. https://doi.org/10.36253/ahsc-8295
Gorni PH, Lima GR, Pereira LMO, Spera KD, Lapaz AM, Pacheco AC (2022) Increasing plant performance, fruit production and nutritional value of tomato through foliar applied rutin. Sci Hortic 294:110755. https://doi.org/10.1016/j.scienta.2021.110755
Gorni PH, Pacheco AC (2016) Growth promotion and elicitor activity of salicylic acid in Achillea millefolium L. Afr J Biotechnol 15:657–665. https://doi.org/10.5897/AJB2016.15320
Gorni PH, Pacheco AC, Silva JFA, Moreli RR, Spera KD, Silva RMG (2019) Plant elicitation with salicylic acid increases bioactive compounds content and antioxidante activity in the infusion of Achillea millefolium L. Biosci J 35:289–295. https://doi.org/10.14393/BJ-v35n1a2019-41788
Gorni PH, Pacheco AC, Lima Moro A, Silva JFA, Moreli RR, de Miranda GR, Pelegrini JM, Spera KD, Bronzel JL Jr, Silva RMG (2020) Salicylic acid foliar application increases biomass, nutrient assimilation, primary metabolites and essential oil content in Achillea millefolium L. Sci Hortic 270:109436. https://doi.org/10.1016/j.scienta.2020.109436
Gorni PH, Pacheco AC, Lima Moro A, Silva JFA, Moreli RR, de Miranda GR, Pelegrini JM, Zaniboni CB, Spera KD, Bronzel JL Jr, Silva RMG (2021) Elicitation improves the leaf area, enzymatic activities, antioxidant activity and content of secondary metabolites in Achillea millefolium grown in the field. J Plant Growth Regul 40:1652–1666. https://doi.org/10.1007/s00344-020-10217-x
Ibrahim A, Abdel-Razzak H, Wahb-Allah M, Alenazi M, Alsadon A, Dewir YH (2019) Improvement in growth, yield, and fruit quality of three red sweet pepper cultivars by foliar application of humic and salicylic acids. HortTechnology 29:170–178. https://doi.org/10.21273/HORTTECH04263-18
Kandoudi W, Németh-Zámboriné E (2022) Stimulating secondary compound accumulation by elicitation: Is it a realistic tool in medicinal plants in vivo? Phytochem Rev 1:19. https://doi.org/10.1007/s11101-022-09822-3
Li J, Wang J, Li J, Liu D, Li H, Gao W, Li J, Liu S (2016) Aspergillus niger enhance bioactive compounds biosynthesis as well as expression of functional genes in adventitious roots of Glycyrrhiza uralensis Fisch. Appl Biochem Biotechnol 178:576–593. https://doi.org/10.1007/s12010-015-1895-5
Li Z, Lee HW, Liang X, Liang D, Wang Q, Huang D, Ong CN (2018) Profiling of phenolic compounds and antioxidant activity of 12 cruciferous vegetables. Molecules 23:1139. https://doi.org/10.3390/molecules23051139
Lichtenthaler HK (1987) Chlorophyll and carotenoids: pigments of photosynthetic biomembranes. In: Packer L, Douce R (eds) Methods Enzymology. Academic Press, Sandiego, pp 350–382
Mazaro SM, Borsatti FC, Dalacosta NL, Lewandowski A, Danner MA, Busso C, Junior AW (2015) Salicylic acid operates in maintenance of post-harvest quality acerolas. Rev Bras Cienc Agrar 10:512–517. https://doi.org/10.5039/agraria.v10i4a5190
Mohamed HI, El-Shazly HH, Badr A (2020) Role of salicylic acid in biotic and abiotic stress tolerance in plants. In: Lone R, Shuab R, Kamili A (eds) Plant Phenolics in Sustainable Agriculture. Springer, Singapore, pp 533–554
Mahmood N, Abbasi NA, Hafiz I, Ali I, Zakia S (2017) Effect of biostimulants on growth, yield and quality of bell pepper cv Yolo Wonder. Pakistan J Agr Sci 54:311–317. https://doi.org/10.21162/PAKJAS/17.5653
Nilsson T (1970) Studies into the pigments in beetroot (Beta vulgaris L. vulgaris var. rubra L.). Lantbrukshogskolans Annaler 36:179–219
Oliveira SPA, do Nascimento HMA, de Sampaio KB, Souza EL (2021) A review on bioactive compounds of beet (Beta vulgaris L. subsp. vulgaris) with special emphasis on their beneficial effects on gut microbiota and gastrointestinal health. Crit Rev Food Sci Nutr 61:2022–2033. https://doi.org/10.1080/10408398.2020.1768510
Pacheco AC, Gorni PH (2021) Elicitation with Salicylic Acid as a Tool for Enhance Bioactive Compounds in Plants. In: Hayat S, Siddiqui H, Damalas CA (eds) Salicylic Acid-A Versatile Plant Growth Regulator. Springer, Cham, pp 1–15. https://doi.org/10.1007/978-3-030-79229-9_1
Panghal A, Virkar K, Kumar V, Dhull SB, Gat Y, Chhikara N (2017) Development of Probiotic Beetroot Drink. Curr Res Nutr Food Sci 5:257–262. https://doi.org/10.12944/CRNFSJ.5.3.10
Preciado-Rangel P, Reyes-Pérez JJ, Ramírez-Rodríguez SC, Salas-Pérez L, Fortis-Hernández M, Murillo-Amador B, Troyo-Diéguez E (2019) Foliar aspersion of salicylic acid improves phenolic and flavonoid compounds, and also the fruit yield in cucumber (Cucumis sativus L.). Plants 8:44. https://doi.org/10.3390/plants8020044
R Core Team (2018) R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna. https://www.R-project.org/
Rahimi P, Abedimanesh S, Mesbah-Namin SA, Ostadrahimi A (2019) Betalains, the nature-inspired pigments, in health and diseases. Crit Rev Food Sci Nutr 59:2949–2978. https://doi.org/10.1080/10408398.2018.1479830
Rathore S, Kumar R (2021) Agronomic interventions affect the growth, yield, and essential oil composition of German chamomile (Matricaria chamomilla L) in the western Himalaya. Ind Crops Prod 171:113873. https://doi.org/10.1016/j.indcrop.2021.113873
Rhaman MS, Rauf F, Tania SS, Karim MM, Sagar A, Robin AHK, Murata Y (2021) Seed priming and exogenous application of salicylic acid enhance growth and productivity of okra (Abelmoschus esculentus L.) by regulating photosynthetic attributes. J Exp Biol Agric Sci 9:759–769. https://doi.org/10.18006/2021.9(6).759.769
Sadowska-Bartosz I, Bartosz G (2021) Biological properties and applications of betalains. Molecules 26:2520. https://doi.org/10.3390/molecules26092520
Sadler G, Davis J, Dezman D (1990) Rapid extraction of lycopene and β-carotene from reconstituted tomato paste and pink grapefruit homogenates. J Food Sci 55:1460–1461. https://doi.org/10.1111/j.1365-2621.1990.tb03958.x
Stagos D, Portesis N, Spanou C, Mossialos D, Aligiannis N, Chaita E, Panagoulis C, Reri E, Skaltsounis L, Tsatsaki AM, Kouretas D (2012) Correlation of total polyphenolic content with antioxidant and antibacterial activity of 24 extracts from Greek domestic Lamiaceae species. Food Chem Toxicol 50:4115–4124. https://doi.org/10.1016/j.fct.2012.08.033
Tajik S, Zarinkamar F, Soltani BM, Nazari M (2019) Induction of phenolic and flavonoid compounds in leaves of saffron (Crocus sativus L.) by salicylic acid. Sci Hortic 257:108751. https://doi.org/10.1016/j.scienta.2019.108751
van Raij B, de Andrade JC, Cantarella H, Quaggio JA (2001) Chemical analysis for evaluation of fertility of tropical soils. Campinas, Instituto Agronômico de Campinas - IAC
Venegas-Molina J, Proietti S, Pollier J, Orozco-Freire W, Ramirez-Villacis D, Leon-Reyes A (2020) Induced tolerance to abiotic and biotic stresses of broccoli and Arabidopsis after treatment with elicitor molecules. Sci Rep 10:1–17. https://doi.org/10.1038/s41598-020-67074-7
Yao X, Zhu L, Chen Y, Tian J, Wang Y (2013) In vivo and in vitro antioxidant activity and α-glucosidase, α-amylase inhibitory effects of flavonoids from Cichorium glandulosum seeds. Food Chem 139:59–66. https://doi.org/10.1016/j.foodchem.2012.12.045
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Pedro Henrique Gorni; methodology, writing original draft, formal analysis, data curation, editing, visualization and supervision, Lázaro da Silva Gonçalves; collected data, writing original draft, Kamille Daleck Spera; editing, Ana Cláudia Pacheco; editing, Allan de Marcos Lapaz; software, formal analysis, editing.
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Gorni, P.H., da Silva Gonçalves, L., Spera, K.D. et al. Exogenous Salicylic Acid Increases Productivity and Elicits Betalains and other Bioactive Compounds in Red Beetroot. Tropical Plant Biol. 16, 41–52 (2023). https://doi.org/10.1007/s12042-023-09329-x
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DOI: https://doi.org/10.1007/s12042-023-09329-x