Skip to main content
SpringerLink
Log in

Induction of polyploidy through colchicine in cotton (Gossypium herbaceum) and its conformity by cytology and flow cytometry analyses

  • Research Article
  • Published:
Journal of Genetics Aims and scope Submit manuscript

Abstract

Cotton is one of the most important fibre crops in the world. An increase in ploidy level was observed in diploid cotton species namely Gossypium herbaceum in the experiment, through colchicine application. There were significant growth variations noticed during the induction of polyploidy in the cotton plants depending upon the concentration of colchicine, duration of the treatments and genotypes taken. An increase in the concentration of colchicine or the duration of the treatment had a retardation effect on seed viability in G. herbaceum. The hypocotyls of length between 4 and 8 mm were found to be most responsive to colchicine treatment. The root meristem of G. herbaceum, treated with 0.2 and 0.4% colchicine exhibited the most number of tetraploid cells during 16 h of treatment. The increase in the concentration of colchicine, along with an increase in duration of treatment led to the chromosomal abnormalities in the wild cotton species. Seed treatment for colchicine application, was the most efficient and reliable method when compared to Petri-plate application and cotton swabbing treatments for inducing polyploidy in diploid cotton.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9

Similar content being viewed by others

References

  • Arunee W., Hormchan P. and Rattanadilok N. 2003 Preliminary test of polyploidy induction in cotton (Gossypium arboretum) using colchicine treatment. J. Nat. Sci. 37, 27–32.

    Google Scholar 

  • Dhamaynthi K. P. M. and Gotmare V. 2010 Induction of polyploidy in two diploid wild cotton (G. armourianum and G. aridum). Electron. J. Plant Breed. 1, 966–972.

    Google Scholar 

  • Dolezel J., Greilhuber J. and Jan Suda J. 2007 Estimation of nuclear DNA content in plants using flow cytometry. Nat. Protocols 2, 2233–2244.

    Article  CAS  Google Scholar 

  • Hendrix B. and Stewart J. M. 2005 Estimation of the nuclear DNA content of Gossypium species. Ann. Bot. 95, 789–797.

  • Jena S. N., Srivastava A., Rai K. M., Ranjan A., Singh S. K. and Nisar T. 2012 Development and characterization of genomic and expressed SSRs for levant cotton (Gossypium herbaceum L.). Theor. Appl. Genet. 124, 565–576.

    Article  CAS  Google Scholar 

  • Levan A. 1939 Tetraploidy and octoploidy induced by colchicine in diploid Petunia. Hereditas 25, 109–131.

    Article  Google Scholar 

  • Liu D., Guo X., Lin Z., Nie Y. and Zhang X. 2006 Genetic diversity of Asian cotton (Gossypium arboreum L.) in China evaluated by microsatellite analysis. Genet. Resou. Crop Evol. 53, 1145–1152.

    Article  CAS  Google Scholar 

  • Mehetre S. S. 1993 Distant hybridization in cotton breeding-intergeneric hybridization (an overview). J. Cotton Res. Dev. 7, 179–192.

    Google Scholar 

  • Morozowska M., Czarna A. and Jędrzejczyk I. 2010 Estimation of nuclear DNA content in Nasturtium R. by flow cytometry. Aqua. Bot. 93, 250–253.

    Article  Google Scholar 

  • Olsen R. T., Ranney T. G. and Vilonia Z. 2006 Reproductive behaviour of induced allotetraploid x Chitalpa and in vitro embryo culture of polyploidy progeny. J. Am. Soc. Hort. Sci. 131, 716–724.

    Article  Google Scholar 

  • Omidbaigi R., Yavari S., Hassani M. E. and Yavari S. 2010 Induction of autotetraploidy in dragonhead (Dracocephalum moldavica L.) by colchicine treatment. J. Fruit Ornamental Plant Res. 18, 23–35.

    CAS  Google Scholar 

  • Percival A. E., Wendel J. F. and Stewart J. M. 1999 Taxonomy and germplasm resources. In Cotton origin, history, technology, and production (ed. C. W. Smith and J. T. Cothren), pp. 33–64. John Wiley, New York.

    Google Scholar 

  • Petersen K. K., Hagberg P. and Kristiansen K. 2003 Colchicine and oryzalin mediated chromosome doubling in different genotypes of Miscanthus sinensis. Plant Cell Tissue Organ Cult. 73, 137–146.

    Article  Google Scholar 

  • Rauf S., Khan I. A. and Khan F. A. 2006 Colchicine-induced tetraploidy and changes in allele frequencies in colchicine-treated populations of diploids assessed with RAPD markers in Gossypium arboreum L. Turk. J. Biol. 30, 93–100.

    CAS  Google Scholar 

  • Senchina D. S., Alvarez L., Cronn R. C., Lin B. and Rong J. 2003 Rate variation among nuclear genes and the age of polyploidy plants in Gossypium. Mol. Biol. Evol. 20, 633–643.

    Article  CAS  Google Scholar 

  • Skovsted A. 1935 Some new interspecific hybrids in the genus Gossypium. J. Genet. 30, 447–463.

    Article  Google Scholar 

  • Stanys V., Staniene G. and Siksnianas T. 2004 In vitro induction of ploidy in Ribes. Acta Universitatis Latviensis Biol. 676, 235–237.

    Google Scholar 

  • Wendel J. F. and Cronn R. C. 2003 Polyploidy and the evolutionary history of cotton. Adv. Agron. 78, 139–186.

    Article  Google Scholar 

  • Wendel J. F. and Grover C. E. 2015 Taxonomy and evolution of the cotton genus. The age of polyploidy plants in Gossypium. Mol. Biol. Evol. 20, 633–643.

    Google Scholar 

Download references

Acknowledgements

Centre of Excellence in Biotechnology, Department of Agricultural Biotechnology, Anand Agricultural University, Anand, Gujarat is acknowledged for funding this research. Seeds of all the selected genotypes in the study were procured, grown and maintained at Research Centre for Distant Hybridization in Field and Fruit Crops, Department of Agricultural Biotechnology, AAU, Anand.

Author information

Author notes

  1. BM and AP planned the research idea, the strategy and analysis of the data. BM conducted the wet lab experiment as a part of his master’s thesis research work. BM and KI wrote the manuscript. AP and MV modified the manuscript. All the authors have read and approved the manuscript.

Authors and Affiliations

  1. Department of Agricultural Biotechnology, Anand Agricultural University, Anand, 388 110, India

    Bhaumik Maru, Akarsh Parihar, Kinjal Kulshrestha & Mahesh Vaja

Authors
  1. Bhaumik Maru
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Akarsh Parihar
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kinjal Kulshrestha
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Mahesh Vaja
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kinjal Kulshrestha.

Additional information

Corresponding editor: Manoj Prasad

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Maru, B., Parihar, A., Kulshrestha, K. et al. Induction of polyploidy through colchicine in cotton (Gossypium herbaceum) and its conformity by cytology and flow cytometry analyses. J Genet 100, 52 (2021). https://doi.org/10.1007/s12041-021-01297-z

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s12041-021-01297-z

Keywords

Search

Navigation