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Cloning and expression of AGL19 gene in two Lonicera macranthoides varieties

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Abstract

AGLl9 is an important regulator for flowering in plants and critical in controlling the morphogenesis of flower organs. The full-length cDNAs of AGL19 in conventional Lonicera macranthoides (Lm-AGL19) and the mutant ‘Xianglei’ cultivar (Lm-XL-AGL19) were obtained using rapid amplification of cDNA ends and the expression vectors for Lm-XL-AGL19 were constructed to investigate the roles of AGL19 in the ‘Xianglei’ cultivar. Lm-AGL19 (GenBank: MK419948) and Lm-XL-AGL19 (GenBank: MK419948) were isolated from the conventional variety and ‘Xianglei’ cultivar of L. macranthoides, respectively. Lm-AGL19 is 1274 bp in length, whereas Lm-XL-AGL19 is 1264-bp long, and both include a 654 bp open reading frame, encoding 217 amino acids, which has a highly conserved MADS_MEF2_like domain and a moderately conserved K-box domain, belonging to the type II MADS-box family of genes. Quantitative real-time polymerase chain reaction indicated that the expression levels of these genes at different flowering stages were significantly different, and that the genes were also expressed in stems and leaves. Lm-XL-AGL19 is underexpressed at flowering period 5 that the key time node for corolla expansion and nonexpansion, while LM-AGL19 is overexpressed during this flowering period. AGL19 was speculated to be a functional gene causing different phenotypes in the two L. macranthoides varieties. The successfully constructed plant expression vector provides an experimental reference for further research on the function of this gene and the basis for the excellent phenotype of L. macranthoides ‘Xianglei’.

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Acknowledgements

This work was supported by ‘the Chinese National Natural Science Foundation’ (Grant Nos. 81203007 and 81673546), ‘The Natural Science Foundation of China Hunan Province’ (Grant No. 2017JJ3237), ‘First-class Discipline Project on Chinese Pharmacology of Hunan University of Chinese Medicine’ ([2018] No. 3), ‘A Project Supported by Scientific Research Fund of Hunan Provincial Education Department’ (Grant No. 19A367), ‘Scientific Research Project Fund of Hunan Agriculture and Countryside Department’ ([2019] no. 105). We thank International Science Editing for editing this manuscript.

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Authors and Affiliations

  1. College of Pharmacy, Hunan University of Chinese Medicine, Changsha, 410208, People’s Republic of China

    YU QING LONG, XIA LIU, JUAN ZENG, CAN LI, XIANG DAN LIU & RI BAO ZHOU

  2. Key Laboratory of Germplasm Resources and Standardized Planting of Hunan Large-scale Genuine Medicinal Materials, Changsha, 410208, People’s Republic of China

    YU QING LONG, XIA LIU, JUAN ZENG, CAN LI, XIANG DAN LIU & RI BAO ZHOU

  3. Hunan Traditional Chinese Medicine Piece Standardization and Function Technology Research Center, Changsha, 410208, People’s Republic of China

    XIANG DAN LIU

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  1. YU QING LONG
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  4. CAN LI
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Correspondence to XIANG DAN LIU or RI BAO ZHOU.

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Corresponding editor: Manoj Prasad

Communicated by Manoj Prasad.

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LONG, Y.Q., LIU, X., ZENG, J. et al. Cloning and expression of AGL19 gene in two Lonicera macranthoides varieties. J Genet 100, 13 (2021). https://doi.org/10.1007/s12041-020-01252-4

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  • DOI: https://doi.org/10.1007/s12041-020-01252-4

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