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Deciphering species relationships and evolution in Chenopodium through sequence variations in nuclear internal transcribed spacer region and amplified fragment-length polymorphism in nuclear DNA

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Abstract

Evaluation of sequence variations in the internal transcribed spacer (ITS) region of 19 accessions, comprising of 11 accessions of Chenopodium quinoa, eight accessions of Chenopodium album and 165 retrieved sequences of different species of Chenopodium belonging to subfamily Chenopodioideae revealed a higher intraspecific genetic diversity in Himalayan C. album than that in C. quinoa. ITS and amplified fragment-length profiles of the accessions suggest the existence of accessions of Himalayan C. album as heteromorphs of the same species rather than a heterogenous assemblage of taxa. While the evolutionary relationship reconstructed from variations in 184 sequences of ITS region from species belonging to Chenopodiaceae, Amaranthaceae, Polygonaceae and Nelumbonaceae established a paraphyletic evolution of family Chenopodiaceae, it also revealed a monophyletic evolution of Chenopodieae I. The reconstruction also established five independent lineages of the subfamily Chenopodioideae with C. album as a sister clade of C. quinoa within the tribe Chenopodieae I. The results also indicate a much younger age for Himalayan chenopods (C. album) than the reported crown age of Chenopodieae I.

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Acknowledgements

Financial support received from Department of Biotechnology, Govt. of India vide grant no. BT/PR-8953/BCE/08/533/2007 and grant no BT/04/NE/2009 under the Biotech Hub programme is gratefully acknowledged. RJD gratefully acknowledges the receipt of financial support from Department of Science & Technology, Govt. of India in the form of a research fellowship under INSPIRE

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  1. Rajkumari Jashmi Devi

    Present address: Institute of Bioresources and Sustainable Development, Takyelpat, Imphal, 795 001, India

Authors and Affiliations

  1. Department of Botany, Centre for Advanced Studies, North-Eastern Hill University, Shillong, 793022, India

    Nikhil K. Chrungoo & Rajkumari Jashmi Devi

  2. Department of Botany, University of Delhi, Delhi, 110 007, India

    Shailendra Goel & Kamal Das

Authors
  1. Nikhil K. Chrungoo
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  2. Rajkumari Jashmi Devi
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Correspondence to Nikhil K. Chrungoo.

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Corresponding Editor: Manoj Prasad.

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Chrungoo, N.K., Jashmi Devi, R., Goel, S. et al. Deciphering species relationships and evolution in Chenopodium through sequence variations in nuclear internal transcribed spacer region and amplified fragment-length polymorphism in nuclear DNA. J Genet 98, 37 (2019). https://doi.org/10.1007/s12041-019-1079-0

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