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Identification of a human ABCC10 orthologue in Catharanthus roseus reveals a U12-type intron determinant for the N-terminal domain feature

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Abstract

ABC (ATP-binding cassette) transporters are members of a large superfamily of proteins that utilize ATP hydrolysis to translocate a wide range of substrates across biological membranes. In general, members of C subfamily (ABCC) are structurally characterized by an additional (N-terminal) transmembrane domain (TMD0). Phylogenetic analysis of plant ABCCs separates their protein sequences into three distinct clusters: I and II are plant specific whereas cluster III contains both human and plant ABCCs. Screening of the Plant Medicinal Genomics Resource database allowed us to identify 16 ABCCs partial sequences in Catharanthus roseus; two of which belong to the unique CrABCC1 transcript that we identified in cluster III. Genomic organization of CrABCC1 TMD0 coding sequence displays an AT-AC U12-type intron that is conserved in higher plant orthologues. We showed that CrABCC1, like its human orthologue ABCC10, produces alternative transcripts that encode protein sequences with a truncated form of TMD0 without the first transmembrane span (TM1). Subcellular localization of CrABCC1 TMD0 variants using yellow fluorescent protein fusions reveals that the TM1 is required for a correct routing of the TMD0 to the tonoplast. Finally, the specific repartition of CrABCC1 orthologues in some species suggests that this gene was lost several times during evolution and that its physiological function may, rely on a common feature of multicellular eukaryotes.

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Acknowledgements

We would like to thank Marc Rideau and Christelle Dutilleul for the critical reading of this paper. This work was partly supported by grants from the Tunisian Ministry of Higher Education and Scientific Research, from the French Institute of Cooperation and from French Ministère de la Recherche et de l’Enseignement Supérieur.

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Authors and Affiliations

  1. EA2106, Biomolécules et Biotechnologies Végétales, Université François Rabelais de Tours, UFR Sciences et Techniques, Parc Grandmont 31, Avenue Monge, 37200, Tours, France

    TAISSIR EL GUIZANI, CLOTILDE GUIBERT, BENOIT ST-PIERRE & ERIC DUCOS

  2. Laboratoire de Biochimie, Département de Biologia, Faculté des Sciences de Tunis, Université Tunis El-Manar, 2092, Tunis, Tunisia

    TAISSIR EL GUIZANI & SAÏDA TRIKI

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  1. TAISSIR EL GUIZANI
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  2. CLOTILDE GUIBERT
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[El-Guizani T., Guibert C., Triki S., St-Pierre B. and Ducos E. 2014 Identification of a human ABCC10 orthologue in Catharanthus roseus reveals a U12-type intron determinant for the N-terminal domain feature. J. Genet. 93, xx–xx]

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GUIZANI, T.E., GUIBERT, C., TRIKI, S. et al. Identification of a human ABCC10 orthologue in Catharanthus roseus reveals a U12-type intron determinant for the N-terminal domain feature. J Genet 93, 21–33 (2014). https://doi.org/10.1007/s12041-014-0327-6

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