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Comprehensive transcriptomics and proteomics analyses of rice stripe virus-resistant transgenic rice

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Abstract

Stable transgenic rice line (named KRSV-1) with strong resistance against rice stripe virus was generated using the gene sequence of disease-specific protein by RNA interference. Comprehensive safety assessment of transgenic plants has turned into a significant field of genetic modification food safety. In this study, a safety assessment of KRSV-1 was carried out in a stepwise approach. The molecular analysis exhibited that KRSV-1 harbored one copy number of transgene, which was integrated into the intergenic non-coding region of chromosome 2 associated with inter-chromosomal translocations of 1.6-kb segments of chromosome 8. Then, transcriptomics and proteomics analyses were carried out to detect the unintended effects as a result of the integration of the transgene. Although 650 dramatically differentially expressed genes (DDEGs) and 357 differentially expressed proteins were detected between KRSV-1 and wild-type (WT) by transcriptomics and proteomics analyses, no harmful members in the form of toxic proteins and allergens were observed. Encouragingly, the nutritional compositions of seeds from KRSV-1 were comparable with WT seeds. The results of this entire study of molecular analysis, transcriptome and proteome profile of KRSV-1 revealed that no detrimental changes in the form of toxic proteins and allergens were detected in the transgenic rice line due to the integration of the transgene.

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Acknowledgements

The authors acknowledge Shandong Entry-Exit Inspection and Quarantine Bureau, Qingdao, China for composition analysis. The authors acknowledge financial support from the China National Transgenic Plant Research and Commercialization Project (Grant No. 2016ZX08001-002) in China.

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Authors and Affiliations

  1. State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai’an, 271018, Shandong, People’s Republic of China

    Yang Xu, Lvjie Bi, Zipeng Yu, Liming Gan, Lifei Zhu, Haiyang Li, Yunzhi Song & Changxiang Zhu

  2. Shandong Peanut Research Institute, Shandong Academy of Agricultural Sciences, Qingdao, 266100, Shandong, People’s Republic of China

    Yang Xu

  3. ShanDong Entry-Exit Inspection and Quarantine Bureau, Qingdao, 266500, Shandong, People’s Republic of China

    Chao Lin

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  1. Yang Xu
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  2. Lvjie Bi
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  3. Zipeng Yu
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  5. Liming Gan
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  6. Lifei Zhu
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Correspondence to Changxiang Zhu.

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Communicated by BJ Rao.

Corresponding editor: BJ Rao

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Xu, Y., Bi, L., Yu, Z. et al. Comprehensive transcriptomics and proteomics analyses of rice stripe virus-resistant transgenic rice. J Biosci 44, 81 (2019). https://doi.org/10.1007/s12038-019-9914-2

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