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Regeneration and evaluation of somaclones of sugarcane variety Co86032 for yellow leaf disease resistance and yield traits

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Abstract

The present investigation was focussed on regeneration, evaluation and screening of somaclones for yellow leaf disease (YLD) resistance using in vitro mutagenesis from a popular susceptible sugarcane variety Co86032 using four chemical mutagens at three levels of concentration (sodium azide (SA) at 0.5 mg L−1, 1.0 mg L−1, 1.5 mg L−1; sodium nitrite (SN) at 3 mg L−1, 5 mg L−1, 7 mg L−1; ethyl methane sulphonate (EMS) at 0.6 μM L−1, 0.8 μM L−1, 1.0 μM L−1 and 2,4 D at 4 mg L−1, 5 mg L−1, 6 mg L−1). A total of 1138 tissue culture seedlings obtained were evaluated for virus resistance both in natural field conditions and in controlled greenhouse condition after aphid vector transmission and presence or absence of virus was observed by visual screening and reverse transcription-polymerase chain reaction method. Four out of 207 asymptomatic plants (16T22, 16T23, 16T29 and 16T31) were devoid of virus coat protein band and were considered to be YLD resistant. The obtained resistance somaclones showed inferior yield traits so they have to be exploited as parents in hybridization programmes with commercial varieties to impart YLD resistance ultimately yielding agronomically superior YLD-resistant varieties in sugarcane.

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Acknowledgements

The authors acknowledge Agricultural Research Station, Perumallapalli, staff for providing the lab facilities to carry out the research programme. They also acknowledge Acharya N.G. Ranga Agricultural University, Andhra Pradesh.

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Authors and Affiliations

  1. Crop Improvement Unit, ICAR-DGR, Junagadh, India

    Praveen Kona

  2. Agricultural Research Station, Perumallapalle, India

    M Hemanth Kumar & T M Hemalatha

  3. Department of Genetics and Plant Breeding, S.V. Agricultural College, Tirupati, India

    K H P Reddy & D M Reddy

  4. Institute of Frontier Technologies, RARS, Tirupati, India

    N P Eswar Reddy & P Latha

Authors
  1. Praveen Kona
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  2. M Hemanth Kumar
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  3. K H P Reddy
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  4. T M Hemalatha
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  5. D M Reddy
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  6. N P Eswar Reddy
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  7. P Latha
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Correspondence to Praveen Kona.

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Communicated by BJ Rao.

Corresponding editor: BJ Rao

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Kona, P., Kumar, M.H., Reddy, K.H.P. et al. Regeneration and evaluation of somaclones of sugarcane variety Co86032 for yellow leaf disease resistance and yield traits. J Biosci 44, 29 (2019). https://doi.org/10.1007/s12038-019-9846-x

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  • DOI: https://doi.org/10.1007/s12038-019-9846-x

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