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Characterization of a DUF820 family protein Alr3200 of the cyanobacterium Anabaena sp. strain PCC7120

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Abstract

The hypothetical protein ‘Alr3200’ of Anabaena sp. strain PCC7120 is highly conserved among cyanobacterial species. It is a member of the DUF820 (Domain of Unknown Function) protein family, and is predicted to have a DNase domain. Biochemical analysis revealed a Mg(II)-dependent DNase activity for Alr3200 with a specific activity of 8.62×104 Kunitz Units (KU) mg−1 protein. Circular dichroism analysis predicted Alr3200 to have ~40% β-strands and ~9% α-helical structures. Anabaena PCC7120 inherently expressed Alr3200 at very low levels, and its overexpression had no significant effect on growth of Anabaena under control conditions. However, Analr3200 +, the recombinant Anabaena strain overexpressing Alr3200, exhibited zero survival upon exposure to 6 kGy of γ-radiation, which is the LD50 for wild type Anabaena PCC7120 as well as the vector control recombinant strain, AnpAM. Comparative analysis of the two recombinant Anabaena strains suggested that it is not the accumulated Alr3200 per se, but its possible interactions with the radiation-induced unidentified DNA repair proteins of Anabaena, which hampers DNA repair resulting in radiosensitivity.

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Authors and Affiliations

  1. Molecular Biology Division, Bhabha Atomic Research Centre, Trombay, Mumbai, 400 085, India

    Prashanth S Raghavan & Hema Rajaram

  2. Protein Crystallography Section, Solid State Physics Division, Bhabha Atomic Research Centre, Trombay, Mumbai, 400 085, India

    Gagan D Gupta & Vinay Kumar

  3. Homi Bhabha National Institute, Training School Complex, Anushaktinagar, Mumbai, 400 094, India

    Hema Rajaram & Vinay Kumar

Authors
  1. Prashanth S Raghavan
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  2. Gagan D Gupta
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  3. Hema Rajaram
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  4. Vinay Kumar
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Corresponding authors

Correspondence to Gagan D Gupta or Hema Rajaram.

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Corresponding editor: B JAGADEESHWAR RAO

[Raghavan PS, Gupta GD, Rajaram H and Kumar V 2016 Characterization of a DUF820 family protein Alr3200 of the cyanobacterium Anabaena sp. strain PCC7120. J. Biosci.]

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Raghavan, P.S., Gupta, G.D., Rajaram, H. et al. Characterization of a DUF820 family protein Alr3200 of the cyanobacterium Anabaena sp. strain PCC7120. J Biosci 41, 589–600 (2016). https://doi.org/10.1007/s12038-016-9646-5

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  • DOI: https://doi.org/10.1007/s12038-016-9646-5

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