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Microirradiation techniques in radiobiological research

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Abstract

The aim of this work is to review the uses of laser microirradiation and ion microbeam techniques within the scope of radiobiological research. Laser microirradiation techniques can be used for many different purposes. In a specific condition, through the use of pulsed lasers, cell lysis can be produced for subsequent separation of different analytes. Microsurgery allows for the identification and isolation of tissue sections, single cells and subcellular components, using different types of lasers. The generation of different types of DNA damage, via this type of microirradiation, allows for the investigation of DNA dynamics. Ion microbeams are important tools in radiobiological research. There are only a limited number of facilities worldwide where radiobiological experiments can be performed. In the beginning, research was mostly focused on the bystander effect. Nowadays, with more sophisticated molecular and cellular biological techniques, ion microirradiation is used to unravel molecular processes in the field of radiobiology. These include DNA repair protein kinetics or chromatin modifications at the site of DNA damage. With the increasing relevance of charged particles in tumour therapy and new concepts on how to generate them, ion microbeam facilities are able to address unresolved questions concerning particle tumour therapy.

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Acknowledgements

The authors thank Steven J Smith for carefully reading the manuscript. GAD acknowledges the financial support of the DFG Cluster of Excellence: Munich-Centre for Advanced Photonics (MAP).

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Authors and Affiliations

  1. Department of Radiation Oncology, University of Munich, Schillerstr. 42, 80336, Munich, Germany

    Guido A Drexler

  2. Universidad de Málaga, Andalucía Tech, Facultad de Medicina, Departamento de Radiología y Medicina Física, Bulevar Louis Pasteur 32, 29071, Málaga, España

    Miguel J Ruiz-Gómez

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Correspondence to Guido A Drexler.

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Corresponding editor: Veena K Parnaik

[Drexler GA and Ruiz-Gómez MJ 2015 Microirradiation techniques in radiobiological research. J. Biosci.] DOI 10.1007/s12038-015-9535-3

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Drexler, G.A., Ruiz-Gómez, M.J. Microirradiation techniques in radiobiological research. J Biosci 40, 629–643 (2015). https://doi.org/10.1007/s12038-015-9535-3

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  • DOI: https://doi.org/10.1007/s12038-015-9535-3

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