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Collagen I-induced dendritic cells activation is regulated by TNF-α production through down-regulation of IRF4

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Abstract

Previously we have shown that collagen I enhances the maturation and function of dendritic cells (DCs). Inflammatory mediators such as tumour necrosis factor (TNF)-α, interleukin (IL)-1β and lipopolysaccharide (LPS) are also known to activate DCs. Here we investigated the involvement of TNF-α on the collagen I-induced DCs activation. TNF-a neutralization inhibited collagen I-induced IL-12 secretions by DCs. Additionally, we observed suppression of collagen I-induced costimulatory molecules expression along with down-regulation of genes involved in DCs activation pathway. Furthermore, TNF-α inhibition upon collagen Istimulation up-regulated the expression of interferon regulatory transcription factor IRF4, when compared to collagen I only treated cells. Collectively, our data demonstrate that collagen I induce TNF-α production, which is crucial for the activation and function of DCs, through down-regulation of IRF4, and implicates the importance in development of anti- TNF-α therapeutics for several inflammatory diseases.

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Abbreviations

DC:

dendritic cells

GMCSF:

granulocyte-macrophage colony stimulating factor

IRF4 :

interferon regulatory factor 4

IL:

interleukin

ECM:

major extracellular matrix

MHC:

major histocompatibility complex

Th1:

T-helper type 1

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Acknowledgements

This study was supported by grants from Wonkwang University (2013).

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Authors and Affiliations

  1. Department of Immunology and Institute of Medical Sciences, Chonbuk National University Medical School, Jeonju, Jeonbuk, 561-756, Korea

    Barun Poudel, Hyeon-Hui Ki & Dae-Ki Kim

  2. Department of Oriental Pharmacy, College of Pharmacy, Wonkwang Korean Medicines Research Institute, Wonkwang University, Iksan, Jeonbuk, 570-749, Korea

    Barun Poudel & Young-Mi Lee

Authors
  1. Barun Poudel
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  2. Hyeon-Hui Ki
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  3. Young-Mi Lee
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Correspondence to Young-Mi Lee or Dae-Ki Kim.

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Corresponding editor: Satyajit Rath

[Poudel B, Ki H-H, Lee Y-M and Kim D-K 2015 Collagen I-induced dendritic cells activation is regulated by TNF-α production through down-regulation of IRF4. J. Biosci. 40 1–8] DOI 10.1007/s12038-015-9507-7

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Poudel, B., Ki, HH., Lee, YM. et al. Collagen I-induced dendritic cells activation is regulated by TNF-α production through down-regulation of IRF4 . J Biosci 40, 71–78 (2015). https://doi.org/10.1007/s12038-015-9507-7

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