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Identification of plant defence regulators through transcriptional profiling of Arabidopsis thaliana cdd1 mutant

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Abstract

A sustainable balance between defence and growth is essential for optimal fitness under pathogen stress. Plants activate immune response at the cost of normal metabolic requirements. Thus, plants that constitutively activate defence are deprived of growth. Arabidopsis thaliana mutant constitutive defence without defect in growth and development1 (cdd1) is an exception. The cdd1 mutant is constitutive for salicylic acid accumulation, signalling, and defence against biotrophic and hemibiotrophic pathogens, without having much impact on growth. Thus, cdd1 offers an ideal genetic background to identify novel regulators of plant defence. Here we report the differential gene expression profile between cdd1 and wild-type plants as obtained by microarray hybridization. Expression of several defence-related genes also supports constitutive activation of defence in cdd1. We screened T-DNA insertion mutant lines of selected genes, for resistance against virulent bacterial pathogen Pseudomonas syringae pv. tomato DC3000 (Pst DC3000). Through bacterial resistance, callose deposition and pathogenesis-associated expression analyses, we identified four novel regulators of plant defence. Resistance levels in the mutants suggest that At2g19810 and [rom] At5g05790 are positive regulators, whereas At1g61370 and At3g42790 are negative regulators of plant defence against bacterial pathogens.

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Acknowledgements

We acknowledge the Arabidopsis Biological Resource Center, Ohio State University, USA, for mutant seed lines, and Genotypic Technology, Bangalore, India, for microarray experiment. This work is supported by the Council of Scientific and Industrial Research, India, Grant (No. 38(1358)/13/EMR-II), and UGC grant (F.No. 40-398/2011(SR)) to AKN, and CSIR fellowships to SS and NS.

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  1. Swadhin Swain

    Present address: Institute of Plant Biology, College of Life Science, National Taiwan University, Taipei, 10617, Taiwan, Republic of China

Authors and Affiliations

  1. School of life Sciences, Jawaharlal Nehru University, New Delhi, 110 067, India

    Swadhin Swain, Nidhi Singh & Ashis Kumar Nandi

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  1. Swadhin Swain
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Correspondence to Ashis Kumar Nandi.

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Corresponding editor: Utpal Nath

[Swain S, Singh N and Nandi AK 2015 Identification of plant defence regulators through transcriptional profiling of Arabidopsis thaliana cdd1 mutant. J. Biosci. 40 1–10] DOI 10.1007/s12038-014-9498-9

Supplementary materials pertaining to this article are available on the Journal of Biosciences Website at http://www.ias.ac.in/jbiosci/mar2015/supp/Swain.pdf

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Swain, S., Singh, N. & Nandi, A.K. Identification of plant defence regulators through transcriptional profiling of Arabidopsis thaliana cdd1 mutant. J Biosci 40, 137–146 (2015). https://doi.org/10.1007/s12038-014-9498-9

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