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Inactivation of Tor proteins affects the dynamics of endocytic proteins in early stage of endocytosis

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Abstract

Tor2 is an activator of the Rom2/Rho1 pathway that regulates α-factor internalization. Since the recruitment of endocytic proteins such as actin-binding proteins and the amphiphysins precedes the internalization of α-factor, we hypothesized that loss of Tor function leads to an alteration in the dynamics of the endocytic proteins. We report here that endocytic proteins, Abp1 and Rvs167, are less recruited to endocytic sites not only in tor2 but also tor1 mutants. Furthermore, we found that the endocytic proteins Rvs167 and Sjl2 are completely mistargeted to the cytoplasm in tor1Δtor2 ts double mutant cells. We also demonstrate here that the efficiency of endocytic internalization or scission in all tor mutants was drastically decreased. In agreement with the Sjl2 mislocalization, we found that in tor1Δtor2 ts double mutant cells, as well as other tor mutant cells, the overall PIP2 level was dramatically increased. Finally, the cell wall chitin content in tor2 ts and tor1Δtor2ts mutant cells was also significantly increased. Taken together, both functional Tor proteins, Tor1 and Tor2, are essentially required for proper endocytic protein dynamics at the early stage of endocytosis.

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Acknowledgements

We thank Dr Hall (University of Basel) for providing tor mutant strains (tor1Δ, tor2 ts , tor1Δtor2 ts, and tor2 overexpressing mutant). This work was supported by a National Scientific Foundation Grant 0923024 (to K KIM) and by thesis funding from Missouri State University (B Tenay).

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  1. Department of Biology, Missouri State University, 901 South National Ave, Springfield, MO, 65897, USA

    Brandon Tenay, Evin Kimberlin, Michelle Williams, Juliette Denise, Joshua Fakilahyel & Kyoungtae Kim

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  1. Brandon Tenay
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  2. Evin Kimberlin
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  3. Michelle Williams
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  4. Juliette Denise
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  6. Kyoungtae Kim
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Correspondence to Kyoungtae Kim.

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Corresponding editor: VEENA K PARNAIK

[Tenay B, Kimberlin E, Williams M, Denise J , Fakilahyel J and Kim K 2013 Inactivation of Tor proteins affects the dynamics of endocytic proteins in early stage of endocytosis. J. Biosci. 38 1–11] DOI 10.1007/s12038-013-9326-7

Supplementary materials pertaining to this article are available on the Journal of Biosciences Website at http://www.ias.ac.in/jbiosci/jun2013/supp/tenay.pdf

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Tenay, B., Kimberlin, E., Williams, M. et al. Inactivation of Tor proteins affects the dynamics of endocytic proteins in early stage of endocytosis. J Biosci 38, 351–361 (2013). https://doi.org/10.1007/s12038-013-9326-7

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