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Changes in leaf proteome profile of Arabidopsis thaliana in response to salicylic acid

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Abstract

Salicylic acid (SA) has been implicated in determining the outcome of interactions between many plants and their pathogens. Global changes in response to this phytohormone have been observed at the transcript level, but little is known of how it induces changes in protein abundance. To this end we have investigated the effect of 1 mM SA on soluble proteins of Arabidopsis thaliana leaves by proteomic analysis. An initial study at transcript level has been performed on temporal landscape, which revealed that induction of most of the SA-responsive genes occurs within 3 to 6 h post treatment (HPT) and the expression peaked within 24 HPT. Two-dimensional gel electrophoresis (2-DE) coupled with MALDI-TOF MS/MS analysis has been used to identify differentially expressed proteins and 63 spots have been identified successfully. This comparative proteomic profiling of SA treated leaves versus control leaves demonstrated the changes of many defence related proteins like pathogenesis related protein 10a (PR10a), disease-resistance-like protein, putative late blight-resistance protein, WRKY4, MYB4, etc. along with gross increase in the rate of energy production, while other general metabolism rate is slightly toned down, presumably signifying a transition from ‘normal mode’ to ‘defence mode’.

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Acknowledgements

We acknowledge Department of Science and Technology (DST), and Council of Scientific and Industrial Research (CSIR), India, for necessary funding. Research activities have been supported by fellowships to RS and RD from CSIR, and ICMR, India, respectively. Central Proteomic facility of CSIR-IICB is acknowledged herewith.

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Authors and Affiliations

  1. Plant Biology Laboratory, Drug Development/Diagnostics & Biotechnology Division, CSIR- Indian Institute of Chemical Biology, Kolkata, 700 032, India

    Riddhi Datta, Ragini Sinha & Sharmila Chattopadhyay

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  1. Riddhi Datta
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  2. Ragini Sinha
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  3. Sharmila Chattopadhyay
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Correspondence to Sharmila Chattopadhyay.

Additional information

Corresponding editor: UTPAL NATH

MS received 27 November 2012; accepted 04 February 2013

Corresponding editor: Utpal Nath

[Datta R, Sinha R and Chattopadhyay S 2013 Changes in leaf proteome profile of Arabidopsis thaliana in response to salicylic acid. J. Biosci. 38 1–12] DOI 10.1007/s12038-013-9308-9

Supplementary materials pertaining to this article are available on the Journal of Biosciences Website at http://www.ias.ac.in/jbiosci/jun2013/supp/datta.pdf

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Datta, R., Sinha, R. & Chattopadhyay, S. Changes in leaf proteome profile of Arabidopsis thaliana in response to salicylic acid. J Biosci 38, 317–328 (2013). https://doi.org/10.1007/s12038-013-9308-9

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