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Distribution Patterns of Subgroups of Inhibitory Neurons Divided by Calbindin 1

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Abstract

The inhibitory neurons in the brain play an essential role in neural network firing patterns by releasing γ-aminobutyric acid (GABA) as the neurotransmitter. In the mouse brain, based on the protein molecular markers, inhibitory neurons are usually to be divided into three non-overlapping groups: parvalbumin (PV), neuropeptide somatostatin (SST), and vasoactive intestinal peptide (VIP)-expressing neurons. Each neuronal group exhibited unique properties in molecule, electrophysiology, circuitry, and function. Calbindin 1 (Calb1), a ubiquitous calcium-binding protein, often acts as a “divider” in excitatory neuronal classification. Based on Calb1 expression, the excitatory neurons from the same brain region can be classified into two subgroups with distinct properties. Besides excitatory neurons, Calb1 also expresses in part of inhibitory neurons. But, to date, little research focused on the intersectional relationship between inhibitory neuronal subtypes and Calb1. In this study, we genetically targeted Calb1-expression (Calb1+) and Calb1-lacking (Calb1−) subgroups of PV and SST neurons throughout the mouse brain by flexibly crossing transgenic mice relying on multi-recombinant systems, and the distribution patterns and electrophysiological properties of each subgroup were further demonstrated. Thus, this study provided novel insights and strategies into inhibitory neuronal classification.

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Data Availability

The datasets during and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

We thank Prof. Miao He of Fudan University for Ai65-F and Ai65 mice.

Funding

This work was supported by the National Natural Science Foundation of China (Grants: 82271486 to XL; 81800133 to AH).

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Author notes

  1. Bing Zhang, Lanfang Li, and Xiaomei Tang contributed equally to this work.

Authors and Affiliations

  1. Institute for Brain Research, Wuhan Center of Brain Science, Huazhong University of Science and Technology, Wuhan, 430030, China

    Bing Zhang, Lanfang Li, Xiaomei Tang, Jinyu Zeng, Yige Song, Zhenye Hou, Henok Kessete Afewerky, Hao Li, Youming Lu, Aodi He & Xinyan Li

  2. Department of Pathophysiology, School of Basic Medicine and Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China

    Bing Zhang, Lanfang Li, Xiaomei Tang, Jinyu Zeng, Yige Song, Zhenye Hou, Henok Kessete Afewerky, Hao Li & Youming Lu

  3. Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China

    Tian Ma

  4. Department of Physiology, School of Basic Medicine and Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China

    Youming Lu

  5. Department of Anatomy, School of Basic Medicine and Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China

    Aodi He & Xinyan Li

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  1. Bing Zhang
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Contributions

XL, AH, and YL conceived and designed the studies. BZ, LL, and XT carried out the experiments, including cell typing, staining and image acquisition, cell counting, and analysis of the data. JZ, YS, ZH, TM, and HL performed the genotyping, electrophysiology, and data analysis experiments. The manuscript was written by HA, AH, and XL. All authors contributed to the article and approved the submitted version.

Corresponding authors

Correspondence to Aodi He or Xinyan Li.

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Mice were bred and reared under the same conditions in accordance with institutional guidelines and the Animal Care and Use Committee of the Animal Core Facility at Huazhong University of Science and Technology. All animal experiments were reviewed and approved by the Medical Ethics Committee at Huazhong University of Science and Technology.

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Zhang, B., Li, L., Tang, X. et al. Distribution Patterns of Subgroups of Inhibitory Neurons Divided by Calbindin 1. Mol Neurobiol 60, 7285–7296 (2023). https://doi.org/10.1007/s12035-023-03542-1

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