Skip to main content

Advertisement

SpringerLink
Log in

Casein release and characterization of electrospun nanofibres for cartilage tissue engineering

  • Published:
Bulletin of Materials Science Aims and scope Submit manuscript

Abstract

Cartilage regeneration/reconstruct is a noticeable challenge in cartilage tissue engineering. In this study, through electrospinning technique, poly(caprolactone) (PCL), gelatin (Gel) and casein (Cas) were used to fabricate electrospun scaffold for cartilage tissue engineering applications. The physicochemical properties of fabricated scaffolds were assessed by scanning electron microscopy (SEM), water contact angle (WCA), mechanical assessment, water uptake measurement and in-vitro degradation assay. The cell/scaffold interactions were assessed through MTT assay and SEM observation. The obtained results showed the uniform and bead-free PCL/Gel/Cas fibres produced with a mean diameter of about 259.27 ± 56.33 nm, and 15.98 ± 2.14 µm pore size. The tensile assessment revealed that PCL/Gel fibres with 2 wt% Cas reached 9.8 ± 1.6 MPa. The MTT assay also showed that PCL/Gel fibres with 2 wt% Cas had proper cell viability and proliferation comparable with PCL/Gel scaffold. It can be concluded that the PCL/Gel/Cas nanofibrous scaffold can be a desirable candidate for cartilage tissue engineering.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6

Similar content being viewed by others

References

  1. Yang R, Chen F, Guo J, Zhou D and Luan S 2020 Bioactive Mater. 5 990

    Article  Google Scholar 

  2. Bigham A, Aghajanian A H, Movahedi M, Sattary M, Rafienia M and Tayebi L 2021 J. Eur. Ceram. Soc. 41 941

    Article  CAS  Google Scholar 

  3. Ghasemiomran V, Movahedi M, Alizadeh F, Khalvati L and Pourahmadi D 2018 Plant Pro. Tech. 10 137

    Google Scholar 

  4. Ding H, Cheng Y, Niu X and Hu Y Pol 2020 J. Biomaterials Sci., (Polymer Edn.) 1

  5. Lin H, Yin C, Mo A and Hong G 2021 Materials 14 235

    Article  CAS  Google Scholar 

  6. Movahedi M, Ghasemiomran V and Torabi S 2016 Ir. J. Med. Arom. Plants 32 758

    Google Scholar 

  7. Movahedi M, Ghasemi-Omran V and Torabi S 2015 J. Plant Mol. Breeding 3 20

    Google Scholar 

  8. Movahedi M, Ghasemiomran V and Torabi S 2016 Ir. J. Med. Arom. Plants 32 32

    Google Scholar 

  9. De Moor L, Beyls E and Declercq H 2020 Ann. Biomed. Eng. 48 298

    Article  Google Scholar 

  10. Rogina A, Pušić M, Štefan L, Ivković A, Urlić I, Ivanković M et al 2021 Ann. Biomed. Eng. 49 1572

    Article  Google Scholar 

  11. Sahoo D R and Biswal T 2021 SN Appl. Sci. 3 1

    Article  CAS  Google Scholar 

  12. Agheb M, Dinari M, Rafienia M and Salehi H 2017 Mater. Sci. Eng.: C 71 240

    Article  CAS  Google Scholar 

  13. Honarvar A, Karbasi S, Hashemibeni B, Setayeshmehr M, Kazemi M and Valiani A 2020 Mat. Tech. 1 1

    Google Scholar 

  14. Bigham A, Salehi A O M, Rafienia M, Salamat M R, Rahmati S, Raucci M G et al 2021 Mater. Sci. Eng.: C 127 112242

    Article  CAS  Google Scholar 

  15. Salehi A O M, Nourbakhsh M S, Rafienia M, Baradaran-Rafii A and Keshel S H 2020 Int. J. Biol. Macromol. 161 377

    Article  CAS  Google Scholar 

  16. Putri N R E, Wang X, Chen Y, Li X, Kawazoe N and Chen G 2020 Prog. Nat. Sci.: Mater. Int. 30 642

    Article  CAS  Google Scholar 

  17. Wen Y-T, Dai N-T and Hsu S-H 2019 Acta Biomater. 88 301

    Article  CAS  Google Scholar 

  18. Movahedi M, Asefnejad A, Rafienia M and Khorasani M T 2020 Inter. J. Biol. Macromol. 146 627

    Article  CAS  Google Scholar 

  19. Movahedi M, Asefnejad A, Rafienia M and Khorasani M T 2021 J. Mazandaran Uni. Med. Sci. 30 1

    Google Scholar 

  20. Zhuikov V A, Akoulina E A, Chesnokova D V, Wenhao Y, Makhina T K, Demyanova I V et al 2021 Polymers 13 108

    Article  CAS  Google Scholar 

  21. Liu R, Zhang S, Zhao C, Yang D, Cui T, Liu Y et al 2021 Nanoscale Res. Lett. 16 1

    Article  CAS  Google Scholar 

  22. Salehi A O M, Keshel S H, Sefat F and Tayebi L 2021 Mater. Today Commun. 27 102402

    Article  CAS  Google Scholar 

  23. Movahedi M, Salehi A O M, Moezi D and Yarahmadian R 2021 Int. J. Poly. Mater. Poly. Biol. 61 1

    Google Scholar 

  24. Cheng A, Schwartz Z, Kahn A, Li X, Shao Z, Sun M et al 2019 Tissue Eng. Part B Rev. 25 14

    Article  Google Scholar 

  25. Chen J-P and Su C-H 2011 Acta Biomater. 7 234

    Article  CAS  Google Scholar 

  26. Han M-E, Kang B J, Kim S-H, Kim H D and Hwang N S 2017 J. Indus. Eng. Chem. 45 421

    Article  CAS  Google Scholar 

  27. Semitela Â, Girão A F, Fernandes C, Ramalho G, Bdikin I, Completo A et al 2020 J. Biomater. Appl. 35 471

    Article  CAS  Google Scholar 

  28. Nadim A, Khorasani S N, Kharaziha M and Davoodi S M 2017 Mater. Sci. Eng.: C 78 47

    Article  CAS  Google Scholar 

  29. Selvaraj S, Thangam R and Fathima N N 2018 Int. J. Biol. Macromol. 120 1674

    Article  CAS  Google Scholar 

  30. Marcone S, Belton O and Fitzgerald D J 2017 Br. J. Clin. Pharm. 83 152

    Article  CAS  Google Scholar 

  31. Wang H-J, Di L, Ren Q-S and Wang J-Y 2009 Materials 2 613

    Article  CAS  Google Scholar 

  32. Latha M, Lal A, Kumary T and Sreekumar R 2000 Contraception 61 329

    Article  CAS  Google Scholar 

  33. Angammana C J and Jayaram S H 2011 IEEE Trans. Indus. Appl. 47 1109

    Article  CAS  Google Scholar 

  34. Pham Q P, Sharma U and Mikos A G 2006 Biomacromolecules 7 2796

    Article  CAS  Google Scholar 

  35. Soliman S, Sant S, Nichol J W, Khabiry M and Traversa E 2011 J. Biomed. Mater. Res. Part A 96 566

    Article  CAS  Google Scholar 

  36. Jiang T, Kai D, Liu S, Huang X, Heng S, Zhao J et al 2018 Biomaterials 178 281

    Article  CAS  Google Scholar 

  37. Dhandayuthapani B, Krishnan U M and Sethuraman S 2010 J. Biomed. Mater. Res. Part B Appl. Biomater. 94 264

    Google Scholar 

  38. Nylander T and Tiberg F 1999 Colloids Surf. B: Biointerfaces 15 253

    Article  CAS  Google Scholar 

  39. Horne D S 2002 Curr. Opin. Colloid Interface sci. 7 456

    Article  CAS  Google Scholar 

  40. Nazari H, Heirani-Tabasi A, Hajiabbas M, SalimiBani M, Nazari M, PirhajatiMahabadi V et al 2020 J. Cell Biochem. 121 2981

    Article  CAS  Google Scholar 

  41. Ren K, Wang Y, Sun T, Yue W and Zhang H 2017 Mater. Sci. Eng.: C 78 324

    Article  CAS  Google Scholar 

  42. Preeth D R, Saravanan S, Shairam M, Selvakumar N, Raja I S, Dhanasekaran A et al 2021 Eur. J. Pharm. Sci. 160 105768

    Article  CAS  Google Scholar 

  43. He X, Feng B, Huang C, Wang H, Ge Y, Hu R et al 2015 Int. J. Nanomed. 10 2089

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biomaterials and Tissue Engineering, School of Advanced Technologies in Medicine, Isfahan University of Medical Sciences, Isfahan, 88137-33395, Iran

    Mehdi Movahedi

  2. Dental Implants Research Center, Dental Research Institute, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, 88137-33395, Iran

    Mehdi Movahedi

  3. Faculty of New Sciences and Technologies, Semnan University, Semnan, 83138-53641, Iran

    Amin Orash Mahmoud Salehi

  4. Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University, Shahrekord, 14619-88631, Iran

    Fatemeh Poodineh hajipour & Sina Etemad

Authors
  1. Mehdi Movahedi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Amin Orash Mahmoud Salehi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Fatemeh Poodineh hajipour
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Sina Etemad
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Mehdi Movahedi.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Movahedi, M., Salehi, A.O.M., hajipour, F.P. et al. Casein release and characterization of electrospun nanofibres for cartilage tissue engineering. Bull Mater Sci 45, 76 (2022). https://doi.org/10.1007/s12034-021-02654-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s12034-021-02654-5

Keywords

Search

Navigation