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LncRNA DGUOK-AS1 Promotes Cell Progression in Lung Squamous Cell Carcinoma by Regulation of miR-653-5p/SLC6A15 Axis

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Abstract

Long noncoding RNA (lncRNA) plays a key role in regulating cancer development. LncRNA deoxyguanosine kinase antisense RNA 1 (DGUOK-AS1) has been reported as a promoter in tumor. The work was designed to further investigate the mechanism of action of DGUOK-AS1 in lung squamous cell carcinoma (LUSC). DGUOK-AS1 level in LUSC cells was measured using RT-qPCR. Counting Kit-8 assays and colony forming assays were performed to evaluate LUSC cell viability and proliferation. Transwell assays were performed to detect cell migration and invasion. Luciferase reporter and RNA pulldown assays were used to verify the binding capacity of DGUOK-AS1 and miR-653-5p. RNA immunoprecipitation assays were performed to verify the relationship of DGUOK-AS1, miR-653-5p, and SLC6A15. DGUOK-AS1 was highly expressed in LUSC cells. DGUOK-AS1 knockdown suppressed LUSC cell proliferation, migration, and invasion. SLC6A15 was demonstrated to be targeted by miR-653-5p, and DGUOK-AS1 interacted with miR-653-5p to modulate SLC6A15 level in LUSC cells. Overexpression of SLC6A15 reversed the suppressive effects of DGUOK-AS1 knockdown on LUSC cell processes. In conclusion, DGUOK-AS1 promotes malignant behaviors of LUSC cells by upregulating SLC6A15 level through interaction with miR-653-5p.

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Data Availability

The datasets used or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

The authors appreciate all the participants providing supports for this study.

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Authors and Affiliations

  1. Key Laboratory of Cancer Prevention and Therapy, Tianjin, Tianjin’s Clinical Research Center for Cancer, Tianjin Medical University Cancer Institute & Hospital, National Clinical Research Center for Cancer, Tianjin, 300060, China

    Yan Teng, Shixia Li, Lijuan Wei, Chi Zhang, Lijuan Li, Shuang Wang, Jing Zhang, Jinchao Huang, Huan Zhang, Nan Wu & Juntian Liu

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  1. Yan Teng
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  2. Shixia Li
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  3. Lijuan Wei
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  4. Chi Zhang
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  5. Lijuan Li
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  7. Jing Zhang
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  8. Jinchao Huang
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  9. Huan Zhang
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  10. Nan Wu
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  11. Juntian Liu
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Contributions

YT was the main designer of this study. YT, SL, LW, CZ, LL, SW, JZ and JL performed the experiments and analyzed the data. YT, JH, HZ, NW and JL drafted the manuscript. All authors read and approved the final manuscript.

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Correspondence to Juntian Liu.

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Teng, Y., Li, S., Wei, L. et al. LncRNA DGUOK-AS1 Promotes Cell Progression in Lung Squamous Cell Carcinoma by Regulation of miR-653-5p/SLC6A15 Axis. Mol Biotechnol (2024). https://doi.org/10.1007/s12033-024-01088-8

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  • DOI: https://doi.org/10.1007/s12033-024-01088-8

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