Skip to main content

Advertisement

Log in

A Short Review on Current Status and Obstacles in the Sustainable Production of Biohydrogen from Microalgal Species

  • Review Paper
  • Published:
Molecular Biotechnology Aims and scope Submit manuscript

Abstract

Biohydrogen is an economical fuel which has enormous promise as an alternative energy source. The synthesis of biohydrogen can be done more affordably and sustainably using microalgae. For the generation of biohydrogen and the treatment of wastewater, microalgae derived from effluent have been showing very impressive outcomes. In comparison to traditional fuel sources, microalgae have benefits. Microalgae are capable of fixing ambient Carbon dioxide and converting it to carbohydrates, which are subsequently processed biochemically to provide fuel. When compared to terrestrial crops, they require less water and minerals for production. But besides these benefits, there are certain technological restrictions on the scale-up implementations of microalgae bioenergy. In this work, we explored the production of biohydrogen from several types of microalgae. The process of producing biohydrogen is affected by a number of variables, including pH, substrate concentration, the kinds of microalgal species, and others. The most recent studies and difficulties related to each stage of the biohydrogen manufacturing process are outlined. The synthesis of microalgal biohydrogen is improved using promising approaches that are discussed. Also, the specific future direction are covered. The possibility for microalgae-based production of biohydrogen to serve as an environmentally friendly and carbon-free biofuel solution that might handle the impending fuel scarcity was demonstrated. However, additional study is required on both the upstream and downstream processes of the synthesis of biohydrogen.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

Data Availability

Data will be available by made on request.

References

Articles included in this review are marked with an asterisk

  1. Li, S., Li, F., Zhu, X., Liao, Q., Chang, J. S., & Ho, S. H. (2022). Biohydrogen production from microalgae for environmental sustainability. Chemosphere, 291, 132717.

    Article  CAS  PubMed  Google Scholar 

  2. Saravanan, A., Kumar, P. S., Khoo, K. S., Show, P. L., Carolin, C. F., Jackulin, C. F., Jeevanantham, S., Karishma, S., Show, K. Y., Lee, D. J., & Chang, J. S. (2021). Biohydrogen from organic wastes as a clean and environment-friendly energy source: Production pathways, feedstock types, and future prospects. Bioresource Technology, 342, 126021.

    Article  CAS  PubMed  Google Scholar 

  3. *Ahmed, S.F., Mofijur, M., Nahrin, M., Chowdhury, S.N., Nuzhat, S., Alherek, M., Rafa, N., Ong, H.C., Nghiem, L.D. and Mahlia, T.M.I. (2022). Biohydrogen production from wastewater-based microalgae: Progresses and challenges. International Journal of Hydrogen Energy, 47(88), 37321–37342. A possible source of biofuel production is algae-derived from sewage. The variables affecting the synthesis of biohydrogen from algal biomass are explored. The main difficulties in the microalgae-based biohydrogen supply chain are spotlighted.

  4. Karishma, S., Saravanan, A., Kumar, P. S., & Rangasamy, G. (2022). Sustainable production of biohydrogen from algae biomass: Critical review on pretreatment methods, mechanism and challenges. Bioresource Technology. https://doi.org/10.1016/j.biortech.2022.128187

    Article  PubMed  Google Scholar 

  5. Rashid, N., Rehman, M. S. U., Memon, S., Rahman, Z. U., Lee, K., & Han, J. I. (2013). Current status, barriers and developments in biohydrogen production by microalgae. Renewable and Sustainable Energy Reviews, 22, 571–579.

    Article  CAS  Google Scholar 

  6. Pathy, A., Nageshwari, K., Ramaraj, R., Maniam, G.P., Govindan, N. and Balasubramanian, P., 2022. Biohydrogen production using algae: Potentiality, Economics and Challenges. Bioresource Technology, p.127514.

  7. Mandotra, S. K., Sharma, C., Srivastava, N., Ahluwalia, A. S., & Ramteke, P. W. (2021). Current prospects and future developments in algal bio-hydrogen production: A review. Biomass Conversion and Biorefinery. https://doi.org/10.1007/s13399-021-01414-z

    Article  Google Scholar 

  8. El-Qelish, M., Hassan, G. K., Leaper, S., Dessì, P., & Abdel-Karim, A. (2022). Membrane-based technologies for biohydrogen production: A review. Journal of Environmental Management, 316, 115239.

    Article  CAS  PubMed  Google Scholar 

  9. *Ananthi, V., Ramesh, U., Balaji, P., Kumar, P., Govarthanan, M. and Arun, A. (2022). A review on the impact of various factors on biohydrogen production. International Journal of Hydrogen Energy. With anaerobes, bio-hydrogen generation may be done more affordably. The bacteria inoculation that has been base-treated can produce more hydrogen.

  10. Sahrin, N. T., Khoo, K. S., Lim, J. W., Shamsuddin, R., Ardo, F. M., Rawindran, H., Hassan, M., Kiatkittipong, W., Abdelfattah, E. A., Da Wen, Oh., & Cheng, C. K. (2022). Current perspectives, future challenges and key technologies of biohydrogen production for building a carbon-neutral future: A review. Bioresource Technology. https://doi.org/10.1016/j.biortech.2022.128088

    Article  Google Scholar 

  11. Woon, J. M., Khoo, K. S., Akermi, M., Alanazi, M. M., Lim, J. W., Chan, Y. J., Goh, P. S., Chidi, B. S., Lam, M. K., Zaini, J., & Bilad, M. R. (2023). Reviewing biohydrogen production from microalgal cells through fundamental mechanisms, enzymes and factors that engendering new challenges and prospects. Fuel, 346, 128312.

    Article  Google Scholar 

  12. Dulta, K., Adeola, A. O., Ashaolu, S. E., Banji, T. I., & Ighalo, J. O. (2022). Biohydrogen production and its bioeconomic impact: A review. Waste Disposal & Sustainable Energy, 4(3), 219–230.

    Article  Google Scholar 

  13. Singh, H., & Das, D. (2020). Biohydrogen from microalgae. Handbook of microalgae-based processes and products (pp. 391–418). Academic Press.

    Chapter  Google Scholar 

  14. Al-Dailami, A., Ahmad, I., Abdullah, N., Koji, I., & Yuzir, A. (2022). Feasibility and viability of procuring biohydrogen from microalgae: An emerging and sustainable energy resource technology. Journal of Physics: Conference Series., 2259(1), 012014.

    Google Scholar 

  15. Wang, K., Khoo, K. S., Chew, K. W., Selvarajoo, A., Chen, W. H., Chang, J. S., & Show, P. L. (2021). Microalgae: The future supply house of biohydrogen and biogas. Frontiers in Energy Research, 9, 660399.

    Article  Google Scholar 

  16. Nagarajan, D., Dong, C. D., Chen, C. Y., Lee, D. J., & Chang, J. S. (2021). Biohydrogen production from microalgae—Major bottlenecks and future research perspectives. Biotechnology Journal, 16(5), 2000124.

    Article  CAS  Google Scholar 

  17. Ahmed, S. F., Rafa, N., Mofijur, M., Badruddin, I. A., Inayat, A., Ali, M. S., Farrok, O., & Yunus Khan, T. M. (2021). Biohydrogen production from biomass sources: Metabolic pathways and economic analysis. Frontiers in Energy Research, 9, 753878.

    Article  Google Scholar 

  18. Ardo, F. M., Lim, J. W., Ramli, A., Lam, M. K., Kiatkittipong, W., Abdelfattah, E. A., Shahid, M. K., Usman, A., Wongsakulphasatch, S., & Sahrin, N. T. (2022). A review in redressing challenges to produce sustainable hydrogen from microalgae for aviation industry. Fuel, 330, 125646.

    Article  Google Scholar 

  19. **Jimenez-Llanos, J., Ramirez-Carmona, M., Rendón-Castrillón, L. and Ocampo-López, C. (2020). Sustainable biohydrogen production by Chlorella sp. microalgae: A review. International Journal of Hydrogen Energy. 45(15), 8310–8328. Hydrogen and products with great economic interest are produced by Chlorella sp. The synthesis of Bio-H2 will be improved via genetic modification and fermenter design. To make the manufacturing of Bio-H2 at a massive scale commercially viable, studies are needed.

  20. El-Dalatony, M. M., Zheng, Y., Ji, M. K., Li, X., & Salama, E. S. (2020). Metabolic pathways for microalgal biohydrogen production: Current progress and future prospectives. Bioresource Technology, 318, 124253.

    Article  CAS  PubMed  Google Scholar 

  21. Hoham, R. W., & Remias, D. (2020). Snow and glacial algae: A review1. Journal of Phycology, 56(2), 264–282.

    Article  PubMed  PubMed Central  Google Scholar 

  22. Sivagurunathan, P., Kumar, G., Mudhoo, A., Rene, E. R., Saratale, G. D., Kobayashi, T., Xu, K., Kim, S. H., & Kim, D. H. (2017). Fermentative hydrogen production using lignocellulose biomass: An overview of pre-treatment methods, inhibitor effects and detoxification experiences. Renewable and Sustainable Energy Reviews, 77, 28–42.

    Article  CAS  Google Scholar 

  23. Goswami, R. K., Mehariya, S., Obulisamy, P. K., & Verma, P. (2021). Advanced microalgae-based renewable biohydrogen production systems: A review. Bioresource Technology, 320, 124301.

    Article  CAS  PubMed  Google Scholar 

  24. Wang, H., Xu, J., Sheng, L., Liu, X., Lu, Y., & Li, W. (2018). A review on bio-hydrogen production technology. International Journal of Energy Research, 42(11), 3442–3453.

    Article  CAS  Google Scholar 

  25. Pandu, K., & Joseph, S. (2012). Comparisons and limitations of biohydrogen production processes: A review. International Journal of Advances in Engineering & Technology, 2(1), 342.

    Google Scholar 

  26. Skjånes, K., Rebours, C., & Lindblad, P. (2013). Potential for green microalgae to produce hydrogen, pharmaceuticals and other high value products in a combined process. Critical reviews in biotechnology, 33(2), 172–215.

    Article  PubMed  Google Scholar 

  27. Woon, J. M., Khoo, K. S., Al-Zahrani, A. A., Alanazi, M. M., Lim, J. W., Cheng, C. K., Sahrin, N. T., Ardo, F. M., Yi-Ming, S., Lin, K. S., & Lan, J. C. W. (2023). Epitomizing biohydrogen production from microbes: Critical challenges vs opportunities. Environmental Research. https://doi.org/10.1016/j.envres.2023.115780

    Article  PubMed  Google Scholar 

  28. *Mona, S., Kumar, S.S., Kumar, V., Parveen, K., Saini, N., Deepak, B. and Pugazhendhi, A. (2020). Green technology for sustainable biohydrogen production (waste to energy): a review. Science of the Total Environment. 728, 138481. In algae, bioenergy is produced by nitrogenases and hydrogenases. The biohydrogen biorefinery may be effective for lowering manufacturing costs. The difficulties and advancements in producing hydrogen were additionally explored.

  29. Liu, C. H., Chang, C. Y., Cheng, C. L., Lee, D. J., & Chang, J. S. (2012). Fermentative hydrogen production by Clostridium butyricum CGS5 using carbohydrate-rich microalgal biomass as feedstock. International Journal of Hydrogen Energy, 37(20), 15458–15464.

    Article  CAS  Google Scholar 

  30. Wieczorek, N., Kucuker, M. A., & Kuchta, K. (2014). Fermentative hydrogen and methane production from microalgal biomass (Chlorella vulgaris) in a two-stage combined process. Applied Energy, 132, 108–117.

    Article  CAS  Google Scholar 

  31. Stanislaus, M. S., Zhang, N., Yuan, Y., Zheng, H., Zhao, C., Hu, X., Zhu, Q., & Yang, Y. (2018). Improvement of biohydrogen production by optimization of pretreatment method and substrate to inoculum ratio from microalgal biomass and digested sludge. Renewable Energy, 127, 670–677.

    Article  CAS  Google Scholar 

  32. Hwang, J. H., Kabra, A. N., Kim, J. R., & Jeon, B. H. (2014). Photoheterotrophic microalgal hydrogen production using acetate-and butyrate-rich wastewater effluent. Energy, 78, 887–894.

    Article  CAS  Google Scholar 

  33. Chen, C. Y., Chang, H. Y., & Chang, J. S. (2016). Producing carbohydrate-rich microalgal biomass grown under mixotrophic conditions as feedstock for biohydrogen production. International Journal of Hydrogen Energy, 41(7), 4413–4420.

    Article  CAS  Google Scholar 

  34. Sengmee, D., Cheirsilp, B., Suksaroge, T. T., & Prasertsan, P. (2017). Biophotolysis-based hydrogen and lipid production by oleaginous microalgae using crude glycerol as exogenous carbon source. International Journal of Hydrogen Energy, 42(4), 1970–1976.

    Article  CAS  Google Scholar 

  35. Yun, Y. M., Jung, K. W., Kim, D. H., Oh, Y. K., & Shin, H. S. (2012). Microalgal biomass as a feedstock for bio-hydrogen production. International journal of hydrogen energy, 37(20), 15533–15539.

    Article  CAS  Google Scholar 

  36. Yang, Z., Guo, R., Xu, X., Fan, X., & Luo, S. (2011). Fermentative hydrogen production from lipid-extracted microalgal biomass residues. Applied Energy, 88(10), 3468–3472.

    Article  CAS  Google Scholar 

  37. Del Rio-Chanona, E. A., Dechatiwongse, P., Zhang, D., Maitland, G. C., Hellgardt, K., Arellano-Garcia, H., & Vassiliadis, V. S. (2015). Optimal operation strategy for biohydrogen production. Industrial & Engineering Chemistry Research, 54(24), 6334–6343.

    Article  Google Scholar 

  38. Park, J. H., Cheon, H. C., Yoon, J. J., Park, H. D., & Kim, S. H. (2013). Optimization of batch dilute-acid hydrolysis for biohydrogen production from red algal biomass. International Journal of Hydrogen Energy, 38(14), 6130–6136.

    Article  CAS  Google Scholar 

  39. Lakshmikandan, M., & Murugesan, A. G. (2016). Enhancement of growth and biohydrogen production potential of Chlorella vulgaris MSU-AGM 14 by utilizing seaweed aqueous extract of Valoniopsis pachynema. Renewable Energy, 96, 390–399.

    Article  CAS  Google Scholar 

  40. Manoyan, J., Gabrielyan, L., Kozel, N., & Trchounian, A. (2019). Regulation of biohydrogen production by protonophores in novel green microalgae Parachlorella kessleri. Journal of Photochemistry and Photobiology B: Biology, 199, 111597.

    Article  CAS  PubMed  Google Scholar 

  41. Sriyod, K., Reungsang, A., & Plangklang, P. (2021). One-step multi enzyme pretreatment and biohydrogen production from Chlorella sp. biomass. International Journal of Hydrogen Energy, 46(80), 39675–39687.

    Article  CAS  Google Scholar 

  42. Ferreira, A. F., Ortigueira, J., Alves, L., Gouveia, L., Moura, P., & Silva, C. (2013). Biohydrogen production from microalgal biomass: Energy requirement, CO2 emissions and scale-up scenarios. Bioresource Technology, 144, 156–164.

    Article  CAS  PubMed  Google Scholar 

  43. Lunprom, S., Phanduang, O., Salakkam, A., Liao, Q., & Reungsang, A. (2019). A sequential process of anaerobic solid-state fermentation followed by dark fermentation for bio-hydrogen production from Chlorella sp. International Journal of Hydrogen Energy, 44(6), 3306–3316.

    Article  CAS  Google Scholar 

  44. Nayak, B. K., Roy, S., & Das, D. (2014). Biohydrogen production from algal biomass (Anabaena sp. PCC 7120) cultivated in airlift photobioreactor. International Journal of Hydrogen Energy, 39(14), 7553–7560.

    Article  CAS  Google Scholar 

  45. Muhamad, K. N., Sahrin, N. T., Alakeel, R. A., Syed, R., Ardo, F. M., Woon, J. M., Tan, W. N., Cheng, C. K., Zango, Z. U., Ho, C. D., & Lam, S. M. (2023). Low thermal pre-treatment of palm kernel expeller to enhance microalgal hydrogen production. Fuel, 345, 128193.

    Article  CAS  Google Scholar 

  46. Christopher, F. C., Kumar, P. S., Vo, D. V. N., & Joshiba, G. J. (2021). A review on critical assessment of advanced bioreactor options for sustainable hydrogen production. International Journal of Hydrogen Energy, 46(10), 7113–7136.

    Article  Google Scholar 

  47. Torzillo, G., Scoma, A., Faraloni, C., & Giannelli, L. (2015). Advances in the biotechnology of hydrogen production with the microalga Chlamydomonas reinhardtii. Critical reviews in biotechnology, 35(4), 485–496.

    Article  CAS  PubMed  Google Scholar 

  48. Bhunia, B., Basak, B., & Dey, A. (2012). A review on production of serine alkaline protease by Bacillus spp. Journal of Biochemical Technology, 3(4), 448–457.

    CAS  Google Scholar 

  49. Pocha, C. K. R., Chia, W. Y., Kurniawan, T. A., Khoo, K. S., & Chew, K. W. (2023). Thermochemical conversion of different biomass feedstocks into hydrogen for power plant electricity generation. Fuel, 340, 127472.

    Article  CAS  Google Scholar 

  50. Show, K. Y., Yan, Y. G., & Lee, D. J. (2019). Biohydrogen production from algae: perspectives, challenges, and prospects. Biofuels from algae (pp. 325–343). Elsevier.

    Chapter  Google Scholar 

  51. Schenk, P. M., Thomas-Hall, S. R., Stephens, E., Marx, U. C., Mussgnug, J. H., Posten, C., Kruse, O., & Hankamer, B. (2008). Second generation biofuels: High-efficiency microalgae for biodiesel production. Bioenergy Research, 1, 20–43.

    Article  Google Scholar 

  52. Buitrón, G., Carrillo-Reyes, J., Morales, M., Faraloni, C., & Torzillo, G. (2017). Biohydrogen production from microalgae. Microalgae-based biofuels and bioproducts (pp. 209–234). Woodhead Publishing.

    Chapter  Google Scholar 

  53. Khoo, K. S., Ahmad, I., Chew, K. W., Iwamoto, K., Bhatnagar, A., & Show, P. L. (2023). Enhanced microalgal lipid production for biofuel using different strategies including genetic modification of microalgae: A review. Progress in Energy and Combustion Science, 96, 101071.

    Article  Google Scholar 

  54. **Zhang, G., Liu, J., Pan, X., Abed, A.M., Le, B.N., Ali, H.E. and Ge, Y. (2022). Latest avenues and approaches for biohydrogen generation from algal towards sustainable energy optimization: Recent innovations, artificial intelligence, challenges, and future perspectives. International Journal of Hydrogen Energy. The use of renewable energy and a cleaner environment are taken into account. Recent advancements in AI for the production of biohydrogen from algae are discussed.

  55. He, J., & Chen, J. P. (2014). A comprehensive review on biosorption of heavy metals by algal biomass: Materials, performances, chemistry, and modeling simulation tools. Bioresource Technology, 160, 67–78.

    Article  CAS  PubMed  Google Scholar 

  56. Aruwajoye, G. S., Kassim, A., Saha, A. K., & Gueguim Kana, E. B. (2020). Prospects for the improvement of bioethanol and biohydrogen production from mixed starch-based agricultural wastes. Energies, 13(24), 6609.

    Article  CAS  Google Scholar 

  57. Salakkam, A., Sittijunda, S., Mamimin, C., Phanduang, O., & Reungsang, A. (2021). Valorization of microalgal biomass for biohydrogen generation: A review. Bioresource Technology, 322, 124533.

    Article  CAS  PubMed  Google Scholar 

  58. Anwar, M., Lou, S., Chen, L., Li, H., & Hu, Z. (2019). Recent advancement and strategy on bio-hydrogen production from photosynthetic microalgae. Bioresource Technology, 292, 121972.

    Article  CAS  PubMed  Google Scholar 

Download references

Funding

This research is not financially supported by any funding agencies.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to P. Senthil Kumar.

Ethics declarations

Competing Interests

The authors declare that no competing interests in this research article.

Ethical Approval

This article does not contain any studies involving animal or human participants performed by any of the authors.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Rathi, B.S., Kumar, P.S. & Rangasamy, G. A Short Review on Current Status and Obstacles in the Sustainable Production of Biohydrogen from Microalgal Species. Mol Biotechnol (2023). https://doi.org/10.1007/s12033-023-00840-w

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s12033-023-00840-w

Keywords

Navigation