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LncRNA SNHG7 Knockdown Aggravates Hepatic Ischemia–Reperfusion Injury and Promotes Apoptosis in Hemorrhagic Shock Pregnant Rats by Modulating miR-34a-5p/YWHAG Axis

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Abstract

Hemorrhagic shock is a frequent threat to pregnant women, and blood transfusions can contribute to organ damage, including hepatic ischemia–reperfusion (HIR) injury. LncRNA SNHG7 (SNHG7) has been reported to exert an essential role in various diseases, while the effect of SNHG7 on HIR injury induced by hemorrhagic shock and reperfusion in pregnant rats is still unclear. In our study, we examined the function and mechanism of SNHG7 in the progression of HIR injury in pregnant rats. The results showed that SNHG7 expression was low in the hepatic tissues of pregnant rats after the hemorrhagic shock and reperfusion modeling. Knockdown of SNHG7 further aggravated hepatic injury, apoptosis, and oxidative stress induced by hemorrhagic shock and reperfusion during pregnancy. Additionally, SNHG7 was bound directly to miR-34a-5p, and miR-34a-5p inhibitors partially reversed the effect of SNHG7 silencing on models of hemorrhagic shock and reperfusion. Furthermore, YWHAG is a direct target of miR-34a-5p and is negatively regulated by miR-34a-5p mimics. Overexpression of YWHAG effectively eliminated the effect of SNHG7 knockdown on pregnant rats. In summary, this investigation proved that SNHG7 knockdown exacerbated HIR injury after hemorrhagic shock in pregnant rats, and reperfusion might by mediating miR-34a-5p/YWHAG axis, indicating that SNHG7 can serve as a target gene for the treatment of HIR injury caused by hemorrhagic shock and reperfusion during pregnancy.

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Data Availability

The data underlying this article are available in the article. If needed, please contact the corresponding author. The email address is zhujiangang1@126.com.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Wujin Hospital Affiliated with Jiangsu University, Changzhou, 213000, China

    Lixia Han & Jie Tang

  2. Department of Hepato-Biliary-Pancreatic Surgery, Nanjing Medical University Affiliated Changzhou No. 2 People’s Hospital, No. 29, Xinglong Lane, Tianning District, Changzhou, 213000, China

    Sheng Zhu & Jiangang Zhu

  3. Department of Obstetrics and Gynecology, The Wujin Clinical College of Xuzhou Medical University, Changzhou, 213000, China

    Lixia Han & Jie Tang

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  1. Lixia Han
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  2. Jie Tang
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Han, L., Tang, J., Zhu, S. et al. LncRNA SNHG7 Knockdown Aggravates Hepatic Ischemia–Reperfusion Injury and Promotes Apoptosis in Hemorrhagic Shock Pregnant Rats by Modulating miR-34a-5p/YWHAG Axis. Mol Biotechnol 65, 983–996 (2023). https://doi.org/10.1007/s12033-022-00613-x

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