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Knockdown of lncRNA LUCAT1 attenuates sepsis‑induced myocardial cell injury by sponging miR-642a

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Abstract

The heart is one of the most common organs involved in sepsis-induced organ dysfunction and about 50% septic patients complicated with myocardial injury. So far, the molecular mechanisms underlying sepsis-induced cardiac damage remain unclear. In this study we aimed to evaluate the effect of miR-642a on sepsis-induced cardiac injury in vitro and explore the possible lncRNA-microRNA mechanism. We first downloaded GSE101639 to identify differentially expressed genes (DEGs) in sepsis. The expression of miR-642a in LPS-induced H9C2 cells was detected by qRT-PCR. MTT assay, cell migration, flow cytometry analysis, ELISA, qRT-PCR and Western blotting analysis were applied to evaluating the effect of miR-642a mimic on LPS-induced H9C2 cells. The bioinformatics analysis and the rescue experiment were devoted to the underlying mechanism. The results showed miR-642a expression was decreased in septic patients and LPS-induced H9C2 cells. Besides, MiR-642a mimic promoted cell viability and migration, inhibited cell apoptosis of LPS-induced H9C2 cells. Bioinformatics analysis showed miR-642a directly targets with 3’-UTR of ROCK1. Moreover, LUCAT1 regulated ROCK1 expression act as a competing endogenous RNA (ceRNA) for miR-642a. Our data demonstrated that lncRNA LUCAT1 could function via sponging miR-642a to regulate ROCK1 expression in LPS-induced H9C2 cells. And knockdown of lncRNA LUCAT1 could suppress LPS-induced cardiac injury in vitro.

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Acknowledgements

This work was supported by Tianjin Union Medical Center.

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Authors and Affiliations

  1. Department of Critical Care Medicine, Tianjin Union Medical Center, No.190, Jieyuan Road, Hongqiao, Tianjin, 300121, China

    Jing Wang, Shaobin Xin & Youjie Qiao

  2. Department of Critical Care Medicine, Tianjin First Central Hospital, Tianjin, 300192, China

    Rui Yang & Jiawei Jiang

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  1. Jing Wang
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  2. Shaobin Xin
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  3. Rui Yang
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  4. Jiawei Jiang
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Correspondence to Youjie Qiao.

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Wang, J., Xin, S., Yang, R. et al. Knockdown of lncRNA LUCAT1 attenuates sepsis‑induced myocardial cell injury by sponging miR-642a. Mamm Genome 32, 457–465 (2021). https://doi.org/10.1007/s00335-021-09890-4

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  • DOI: https://doi.org/10.1007/s00335-021-09890-4

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