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Cloning and Functional Identification of Gibberellin Receptor SvGID1s Gene of Salix viminalis

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Abstract

Gibberellins (GAs) play a key role in the transition from vegetative growth to flowering and the GA receptor GID1 (GIBBERELLIN INSENSITIVE DWARF1) is the central part of GA-signaling. The differential expression of SvGID1 was found in the transcriptome sequencing in our previous study, which was further verified at different stages of flowering of Salix viminalis. In order to reveal the function GID1 of S. viminalis, two genes of SvGID1b and SvGID1c were cloned and transformed into Arabidopsis thaliana, respectively. The results showed that the full ORF length of SvGID1b and SvGID1c genes were both 1035 bp, encoding 344 amino acids, which were typical globular proteins. The peptide chain contained more α-helix structure, and had 99% similarity with GID1b and GID1c amino acid sequences of Salix suchowensis. Phylogenetic analysis showed that SvGID1s had close genetic relationship with woody plants such as Populus alba and Populus tomentosa, and had far genetic relationship with rice. After overexpression in A. thaliana, the total gibberellin, active gibberellin content and the expression level of GA3ox1, the key gene for GA4 synthesis, were not significantly different from those in the wild-type, while the expression levels of FUL, SOC1 and FT, the key genes for flowering in plants, were increased, and the expression levels of FLC and GAI were decreased. The ectopic expression of SvGID1s increased the sensitivity of plants to gibberellin and enhanced gibberellin effect, caused early bolting, budding and flowering, led to higher plant, longer hypocotyl and other phenomena. The results provide a theoretical basis for clarifying the regulation of gibberellin on flower bud differentiation of flowering plants.

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Acknowledgements

We thank the Shandong Provincial Natural Science Fund (ZR2020MC159) for its support and the 35S-GFP-NOS-1300 plasmid provided by Dr. Song Yuguang of Qufu Normal University.

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Shandong Provincial Natural Science Fund (ZR2020MC159).

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  1. College of Life Science, Qufu Normal University, Qufu, 273165, People’s Republic of China

    Qingwei Liu, YaRu Wu, Xin Zhang, Min Song & Xiangyong Peng

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  1. Qingwei Liu
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QL performed experimental work and data analysis. QL and XP designed the study and contributed to writing and revising the manuscript. YW and XZ contributed to experimental work. MS contributed to revising the manuscript. All authors read and approved the final manuscript.

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Correspondence to Xiangyong Peng.

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Liu, Q., Wu, Y., Zhang, X. et al. Cloning and Functional Identification of Gibberellin Receptor SvGID1s Gene of Salix viminalis. Mol Biotechnol 65, 715–725 (2023). https://doi.org/10.1007/s12033-022-00573-2

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