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Circ_0001982 Up-regulates the Expression of E2F1 by Adsorbing miR-1205 to Facilitate the Progression of Glioma

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Abstract

This study was aimed at probing into the regulatory effects of circular RNA (circRNA)_0001982 on glioma cell proliferation, migration, invasion, and cell cycle, and its underlying mechanism. CircRNA expression profile of glioma tissues/normal brain tissues was downloaded from the Gene Expression Omnibus (GEO) database and analyzed. Circ_0001982, microRNA (miRNA, miR)-1205, and E2F transcription factor 1 (E2F1) expressions in glioma tissues and cell lines were quantified using quantitative real-time polymerase chain reaction (qRT-PCR) and/or Western blot. Glioma cell proliferation, migration, invasion, and cell cycle were detected employing cell counting kit-8 (CCK-8), 5-Ethynyl-2′-deoxyuridine (EdU), scratch-healing, Transwell, and flow cytometry assays, respectively. The targeting relationships between miR-1205 and circ_0001982, and miR-1205 and E2F1 3′UTR were verified using bioinformatics, dual-luciferase reporter experiments, and RNA immunoprecipitation (RIP) assay. Pearson’s correlation analysis was applied to detect the correlations among circ_0001982, miR-1205, and E2F1 expression levels. Circ_0001982 expression level was increased in glioma tissues and correlated with larger tumor size. Circ_0001982 overexpression enhanced glioma cell proliferation, migration, and invasion, and accelerated cell cycle progression while knocking down circ_0001982 exerted opposite effects. Circ_0001982 directly targeted miR-1205, and miR-1205 directly targeted E2F1. Besides, circ_0001982 could up-regulate E2F1 expression via repressing miR-1205 expression. Circ_0001982 accelerates glioma progression by modulating the miR-1205/E2F1 axis.

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Data Availability

The data used to support the findings of this study are available from the corresponding author upon request.

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Acknowledgements

We thank Hubei Yican Health Industry Co., Ltd for its linguistic assistance during the preparation of this manuscript.

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Authors and Affiliations

  1. Department of Neurosurgery, The First Hospital of Qinhuangdao, The First Hospital of Qinhuangdao, Qinhuangdao, Hebei, China

    Zhihui Ma

  2. Department of Neurology, The First College of Clinical Medical Sciences, China Three Gorges University & Yichang Central People’s Hospital, Yichang, Hubei, China

    Jinyang Ma & Bojuan Lang

  3. Department of Neurosurgery, Shenzhen People’s Hospital, The 2nd Clinical Medical College of Jinan University, Shenzhen, 518020, Guangdong, China

    Fei Xu & Bo Zhang

  4. Department of Neurosurgery, The First Affiliated Hospital of Jinan University, Guangzhou, 510630, Guangdong, China

    Xiangyu Wang

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Correspondence to Bojuan Lang.

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Ma, Z., Ma, J., Lang, B. et al. Circ_0001982 Up-regulates the Expression of E2F1 by Adsorbing miR-1205 to Facilitate the Progression of Glioma. Mol Biotechnol 65, 466–476 (2023). https://doi.org/10.1007/s12033-022-00540-x

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