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LncRNA ARAP1-AS1 Promotes Bladder Cancer Development by Regulating the miR-3918/KIF20A Axis

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Abstract

This study is to clarify the effect of the long non-coding RNA ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1-antisense RNA 1 (ARAP1-AS1)/microRNA (miR)-3918/kinesin family member 20A (KIF20A) on bladder cancer cell function. ARAP1-AS1, miR-3918, and KIF20A expression levels in bladder cancer cells were determined using quantitative reverse transcription-polymerase chain reaction. The effects of ARAP1-AS1, miR-3918, and KIF20A on bladder cell activity, proliferation, apoptosis, and in vivo growth were examined using the cell counting kit-8, colony formation, caspase-3 activity, and xenograft tumor growth assays, respectively, in nude mice. The binding relationships among ARAP1-AS1, miR-3918, and KIF20A were analyzed using luciferase and RNA immunoprecipitation assays. ARAP1-AS1 and KIF20A were overexpressed in bladder cancer, while miR-3918 was underexpressed. The downregulation of ARAP1-AS1 or KIF20A expression significantly inhibited the viability and proliferation of cancer cells and promoted apoptosis, whereas low expression of miR-3918 or high expression of ARAP1-AS1/KIF20A showed the opposite effect. miR-3918 was sponged by ARAP1-AS1, and targeted KIF20A. In addition, miR-3918 expression was inversely correlated with ARAP1-AS1 and KIF20a expression levels in bladder cancer tissues. In addition, the rescue experiment showed that interference with miR-3918 could reverse the effect of low ARAP1-AS1 or KIF20A expression on bladder cancer cell malignancy. ARAP1-AS1 facilitates the malignant behavior of bladder cancer cells via the regulation of KIF20A expression by sponging miR-3918.

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Data Availability

The datasets used and/or analyzed in this study are available from the corresponding author on reasonable request.

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Acknowledgements

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Funding

This work was supported by Wuhan Key Project: Research on Molecular Phenotyping Spectrum Identification and Clinical Application Evaluation of Circulating Prostate Cancer Cells [grant number WX18A05].

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Authors and Affiliations

  1. Department of Urology, Wuhan Third Hospital, Tongren Hospital of Wuhan University, No. 216 Guanshan Avenue, Hongshan District, Wuhan, 430014, Hubei, China

    Wei Zhang, Jingyu Zhang, Zhi Hu, Wei Sun, Lv Xu, Hao Chu, Xiao Wang & Qiao Fu

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  1. Wei Zhang
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  2. Jingyu Zhang
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  3. Zhi Hu
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  4. Wei Sun
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  5. Lv Xu
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  6. Hao Chu
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  7. Xiao Wang
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  8. Qiao Fu
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Contributions

WZ and JYZ conducted the experiments and analyzed data. ZH and WS offered the experimental consideration. LX and HC analyzed and interpreted data. XW did the analysis and interpretation of data. QF collected materials and resources. The manuscript was read and approved through all authors.

Corresponding author

Correspondence to Qiao Fu.

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No potential competing interest was reported by the author.

Ethical Approval

Ethics Committee of Wuhan Third Hospital (Wuhan, China) approved the present study. Clinical analysis complied with the ethical standards of the Declaration of Helsinki.

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Written informed consent acquired from all patients.

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Zhang, W., Zhang, J., Hu, Z. et al. LncRNA ARAP1-AS1 Promotes Bladder Cancer Development by Regulating the miR-3918/KIF20A Axis. Mol Biotechnol 64, 1259–1269 (2022). https://doi.org/10.1007/s12033-022-00489-x

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  • DOI: https://doi.org/10.1007/s12033-022-00489-x

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