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Fabrication and Molecular Modeling of Navette-Shaped Fullerene Nanorods Using Tobacco Mosaic Virus as a Nanotemplate

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Abstract

To date, metallization studies have been performed with the nanometer-scale template, Tobacco Mosaic Virus (TMV). Here we show that fullerenes as well can be deposited on TMV coat protein in a controlled manner. Two methods were followed for the coating process. First, underivatized fullerene was dispersed in different solvents to bring the underivatized fullerene and wild-type TMV together. Improved depositions were obtained with the fullerene dicarboxylic derivative synthesized via the Bingel method. The form of the coating was analyzed by transmission electron microscopy. Our results demonstrate that the coating efficiency with the carboxy derivative was much better compared to the underivatized fullerene. The goal of coupling a carbon nanoparticle to a biological molecule, the viral coat of TMV, was achieved with the carboxy derivative of fullerene, resulting in the production of navette-shaped nanorods. The interactions between carboxyfullerenes and TMV were investigated through modeling with computational simulations and Gaussian-based density functional theory (DFT) calculations using the Gaussian09 program package. The theoretical calculations supported the experimental findings. This inexpensive and untroublesome method promises new fullerene hybrid nanomaterials in particular shapes and structures.

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Ankara Üniversitesi Biyoteknoloji Enstitüsü, BIBAP 05/169, Yaşar Murat Elçin

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  1. Tissue Engineering, Biomaterials and Nanobiotechnology Laboratory, Faculty of Science, and Stem Cell Institute, Ankara University, Ankara, Turkey

    Çiğdem Dönmez Güngüneş, Ayşe Eser Elçin & Yaşar Murat Elçin

  2. Department of Nutrition and Dietetics, Faculty of Health Sciences, Hitit University, Corum, Turkey

    Çiğdem Dönmez Güngüneş

  3. Chemistry Department, Faculty of Arts and Sciences, Hitit University, Corum, Turkey

    Sinan Başçeken

  4. Biovalda Health Technologies, Inc, Ankara, Turkey

    Yaşar Murat Elçin

  5. Faculty of Science, Biochemistry Division, Ankara University, Tandogan, 06100, Ankara, Turkey

    Yaşar Murat Elçin

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Y.M.E. is the founder and shareholder of Biovalda Health Technologies, Inc. (Ankara, Turkey). The authors declare no competing financial interests in relation to this particular article. The authors are alone responsible for the content and writing of the paper.

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Dönmez Güngüneş, Ç., Başçeken, S., Elçin, A.E. et al. Fabrication and Molecular Modeling of Navette-Shaped Fullerene Nanorods Using Tobacco Mosaic Virus as a Nanotemplate. Mol Biotechnol 64, 681–692 (2022). https://doi.org/10.1007/s12033-021-00440-6

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