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Clinical Significance and Biological Function of miR-1274a in Non-small Cell Lung Cancer

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Abstract

Since the discovery of microRNAs (miRNAs) as a class of important regulatory molecules, miRNAs are involved in the occurrence and development of tumors. In this paper, we aimed to identify the role of miR-1274a in non-small cell lung cancer (NSCLC). The miR-1274a expression levels in four NSCLC cells and tissues from 125 patients were determined by qRT-PCR assays. Kaplan–Meier survival curves and Cox regression analysis were used to examine the prognostic significance of miR-1274a in NSCLC patients. The CCK-8 and Transwell assays were performed to evaluate the cell proliferation, invasion, and migration ability of NSCLC cells. The miR-1274a expression levels were significantly higher in NSCLC tissues than in adjacent normal tissues, and overexpression of miR-1274a had a poor prognosis in NSCLC patients. Functional studies in two NSCLC cell lines have shown that overexpression of miR-1274a could promote cell proliferation, migration, and invasion. miR-1274a expression levels are upregulated in NSCLC tissues, and a high expression is associated with a poor prognosis in patients with NSCLC. Moreover, miR-1274a promotes cell proliferation, migration, and invasion. Based on our findings, miR-1274a may act as a tumor miRNA in the occurrence and development of NSCLC.

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All data generated or analyzed during this study are included in this published article.

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Funding

This work was supported by scientific research fund of Petrel (Grant No. JJQN2016-11]; the financial assistance of Heilongjiang Postdoctoral Fund (Grant No. LBH-Z16227).

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Authors and Affiliations

  1. Clinical Oncology Center, Hong Kong University Shenzhen Hospital, Shenzhen, 518000, Guangdong, China

    Shi-jia Zhu

  2. Department of Radiation Oncology, The Affiliated Tumor Hospital of Harbin Medical University, No. 150 Haping Road, Nangang District, Harbin, 150040, Heilongjiang, China

    Xiao Wang, Song-liu Hu, Bi-xi Guan, Jian Li, Gen Li & Jian-yu Xu

  3. Department of Phase I Clinical Trial, The Affiliated Tumor Hospital of Harbin Medical University, Harbin, 150040, Heilongjiang, China

    Yu Fang

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  1. Shi-jia Zhu
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Correspondence to Jian-yu Xu.

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This study was approved by the hospital ethics committee of the affiliated Tumor Hospital of Harbin Medical University.

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Zhu, Sj., Wang, X., Hu, Sl. et al. Clinical Significance and Biological Function of miR-1274a in Non-small Cell Lung Cancer. Mol Biotechnol 64, 9–16 (2022). https://doi.org/10.1007/s12033-021-00385-w

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  • DOI: https://doi.org/10.1007/s12033-021-00385-w

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