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Effects of Gentamicin-Loaded Chitosan-ZnO Nanocomposite on Quorum-Sensing Regulation of Pseudomonas Aeruginosa

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Abstract

Cell density-based intercellular signaling mechanism is known as Quorum sensing (QS); it serves a significant role in regulating the pathogenic factors. The objective of the present study was to assess the influence of chitosan-zinc oxide nanocomposite (CH-ZnO nanocomposite), alone and in combination with gentamicin, on the sensitivity to hydrogen peroxide (H2O2), the production of pathogenic factors and QS-regulated genes of Pseudomonas aeruginosa. The efficacy of the minimum inhibitory concentration (MIC) and 1/4 MIC of the CH-ZnO nanocomposite, alone and in combination with gentamicin, on the sensitivity to H2O2, pyocyanin secretion, swarming and twitching motilities was evaluated. In addition, the expression of some QS-regulated genes including rhlI, rhlR, lasI and lasR genes was measured by Real-time quantitative PCR (RT-qPCR) following exposure to the nanocomposite. The results demonstrated that at MIC concentrations, the gentamicin-loaded CH-ZnO nanocomposite significantly inhibited QS-regulated phenotypes such as pyocyanin secretion (82.4%), swarming (76%) and twitching (73.6%) motilities; further it increased the inhibition growth zone (134.5%), as well as, at 1/4 MIC concentration decreased the expression of lasI (72%), lasR (78%), rhlI (76%) and rhlR (82%) genes; as compared to untreated P. aeruginosa PAO1 (P < 0.05). Our results also demonstrated that the CH-ZnO nanocomposite combined with gentamicin could be a potential innovative candidate, which could be broadly applied in the treatment of P. aeruginosa infections.

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Acknowledgements

This work was financially supported by the Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran (Grant No. 97/60073).

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Authors and Affiliations

  1. Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Fatemeh Hemmati, Pourya Gholizadeh, Roghayeh Nouri & Mohammad Ahangarzadeh Rezaee

  2. Department of Medical Microbiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

    Reza Ghotaslou, Hossein Samadi Kafil, Alka Hasani & Mohammad Ahangarzadeh Rezaee

  3. Drug Applied Research Center, Tabriz University of Medical Science, Tabriz, Iran

    Roya Salehi & Hossein Samadi Kafil

  4. Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran

    Fatemeh Hemmati, Pourya Gholizadeh & Roghayeh Nouri

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  1. Fatemeh Hemmati
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Hemmati, F., Ghotaslou, R., Salehi, R. et al. Effects of Gentamicin-Loaded Chitosan-ZnO Nanocomposite on Quorum-Sensing Regulation of Pseudomonas Aeruginosa. Mol Biotechnol 63, 746–756 (2021). https://doi.org/10.1007/s12033-021-00336-5

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