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DNA Fingerprinting and Genetic Relationships Similarities Among the Accessions/Species of Ocimum Using SCoT and ISSR Markers System

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Abstract

Studies on genetic diversity could enhance taxonomic authentication and evolutionary relationship among the species of Ocimum. Therefore, diversity among 36 Ocimum accessions representing species from different regions of world were analyzed using Start Codon-Targeted Polymorphism (SCoT) and inter-simple sequences repeat (ISSR) marker. Marker systems used in this study was potentially targeted the different regions of the genome and included 18 SCoT and 15 ISSR primers, which showed successful amplification profile for Ocimum. Between these two, SCoT revealed the highest mean value of percentage of Polymorphism (84.6%), polymorphic information content (PIC, 0.65), and resolving power (Rp, 8.80), which were higher than ISSR. A total of 140 and 111 amplicons were obtained with SCoT and ISSR marker. The Mantel test indicted a significant correlation (r2 = 0.44) between ISSR and SCoT, which suggested a common genetical background among the accessions. The principal coordinate study showed the selection of different Ocimum genotypes by the cluster analysis. This study will help and support identification, genetic mapping, and molecular ecology to enhance the breeding program's efficiency for developing elite varieties to meet industrial demand globally. The present study is the first report of the genetic diversity, and relationship determination with SCoT-based molecular marker among Ocimum accessions.

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Acknowledgements

We are thankful for support and facilities provided by Director, CSIR-CIMAP, Lucknow, India. PG acknowledges the Academy of Scientific and Innovative Research (AcSIR) for registration of Ph.D. program.

Funding

This work was supported by CSIR- Aroma mission HCP 0007. CIMAP Communication Number CIMAP/PUB/2020/OCT/109.

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Authors and Affiliations

  1. CSIR- Human Resource Development Centre Campus, Academy of Scientific and Innovative Research (AcSIR), Sector 19, Kamla Nehru Nagar, Ghaziabad, UP, 201002, India

    Pankhuri Gupta

  2. Biotechnology Division, CSIR- Central Institute for Medicinal and Aromatic Plants, P.O. CIMAP, Lucknow, UP, 226015, India

    Pankhuri Gupta & Sunita Singh Dhawan

  3. Genetics and Plant Breeding Division, CSIR- Central Institute for Medicinal and Aromatic Plants, Lucknow, UP, 226015, India

    Anand Mishra & R. K. Lal

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  1. Pankhuri Gupta
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PG carried out sample collection, design and execution of data integration, manuscript preparation. AM performed assisted in data and software analysis. RKL helped in field experiments. SSD was responsible for conceptualization, designing of the experiments, data analysis, data integration, drafting and finalization of the manuscript.

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Correspondence to Sunita Singh Dhawan.

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Gupta, P., Mishra, A., Lal, R.K. et al. DNA Fingerprinting and Genetic Relationships Similarities Among the Accessions/Species of Ocimum Using SCoT and ISSR Markers System. Mol Biotechnol 63, 446–457 (2021). https://doi.org/10.1007/s12033-021-00316-9

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