Skip to main content
SpringerLink
Log in

Molecular Cloning and Characterization of O-Methyltransferase from Mango Fruit (Mangifera indica cv. Alphonso)

  • Original Paper
  • Published:
Molecular Biotechnology Aims and scope Submit manuscript

Abstract

Flavour of ripe Alphonso mango is invariably dominated by the de novo appearance of lactones and furanones during ripening. Of these, furanones comprising furaneol (4-hydroxy-2,5-dimethyl-3(2H)-furanone) and mesifuran (2,5-dimethyl-4-methoxy-3(2H)-furanone) are of particular importance due to their sweet, fruity caramel-like flavour characters and low odour detection thresholds. We isolated a 1056 bp complete open reading frame of a cDNA encoding S-adenosyl-l-methionine-dependent O-methyltransferase from Alphonso mango. The recombinantly expressed enzyme, MiOMTS showed substrate specificity towards furaneol and protocatechuic aldehyde synthesizing mesifuran and vanillin, respectively, in an in vitro assay reaction. A semi-quantitative PCR analysis showed fruit-specific expression of MiOMTS transcripts. Quantitative real-time PCR displayed ripening-related expression pattern of MiOMTS in both pulp and skin of Alphonso mango. Also, early and significantly enhanced accumulation of its transcripts was detected in pulp and skin of ethylene-treated fruits. Ripening-related and fruit-specific expression profile of MiOMTS and substrate specificity towards furaneol is a suggestive of its involvement in the synthesis of mesifuran in Alphonso mango. Moreover, a significant trigger in the expression of MiOMTS transcripts in ethylene-treated fruits point towards the transcriptional regulation of mesifuran biosynthesis by ethylene.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  1. Macleod, A. J., & Pieris, N. M. (1984). Comparison of the volatile components of some mango cultivars. Phytochemistry, 23, 361–366.

    Article  CAS  Google Scholar 

  2. Pandit, S. S., Chidley, H. G., Kulkarni, R. S., Pujari, K. H., Giri, A. P., & Gupta, V. S. (2009). Cultivar relationships in mango based on fruit volatile profiles. Food Chemistry, 114, 363–372.

    Article  CAS  Google Scholar 

  3. Pino, J. A., & Mesa, J. (2006). Contribution of volatile compounds to mango (Mangifera indica L.) aroma. Flavour and Fragrance Journal, 21, 207–213.

    Article  CAS  Google Scholar 

  4. Kulkarni, R. S., Chidley, H. G., Pujari, K. H., Giri, A. P., & Gupta, V. S. (2012). Flavor of mango: A pleasant but complex blend of compounds. In K. R. Sudha, G Valavi, J. N Govil, K. V Peter, & G. Thottappilly (Eds.), Mango: Production and processing technology (Vol 1). Houston: Studium Press LLC.

  5. Kulkarni, R. S., Chidley, H. C., Pujari, K. H., Giri, A. P., & Gupta, V. S. (2012). Geographic variation in the flavor volatiles of Alphonso mango. Food Chemistry, 130, 58–66.

    Article  CAS  Google Scholar 

  6. Pandit, S. S., Kulkarni, R. S., Chidley, H. G., Giri, A. P., Pujari, K. H., Kollner, T. G., et al. (2009). Changes in volatile composition during fruit development and ripening of ‘Alphonso’ mango. Journal of the Science of Food and Agriculture, 89, 2071–2081.

    Article  CAS  Google Scholar 

  7. Wilson, C. W., Shaw, P. E., & Knight, R. J. (1990). Importance of some lactones and 2,5-dimethyl-4-hydroxy-3(2H)-furanone to mango (Mangifera indica L.) aroma. Journal of Agricultural and Food Chemistry, 38, 1556–1559.

    Article  CAS  Google Scholar 

  8. Schwab, W., & Roscher, R. (1997). 4-Hydroxy-3(2H)-furanones: Natural and Maillard products. Recent Research Developments in Phytochemistry, 1, 643–673.

    Google Scholar 

  9. Schwab, W. (2013). Natural 4-hydroxy-2,5-dimethyl-3(2H)-furanone (Furaneol(R)). Molecules, 18, 6936–6951.

    Article  CAS  Google Scholar 

  10. Lavid, N., Schwab, W., Kafkas, E., Koch-Dean, M., Bar, E., Larkov, O., et al. (2002). Aroma biosynthesis in strawberry: S-adenosylmethionine: Furaneol O-methyltransferase activity in ripening fruits. Journal of Agricultural and Food Chemistry, 50, 4025–4030.

    Article  CAS  Google Scholar 

  11. Wein, M., Lavid, N., Lunkenbein, S., Lewinsohn, E., Schwab, W., & Kaldenhoff, R. (2002). Isolation, cloning and expression of a multifunctional O-methyltransferase capable of forming 2,5-dimethyl-4-methoxy-3(2H)-furanone, one of the key aroma compounds in strawberry fruits. Plant Journal, 31, 755–765.

    Article  CAS  Google Scholar 

  12. Kulkarni, R., Chidley, H., Deshpande, A., Schmidt, A., Pujari, K., Giri, A., et al. (2013). An oxidoreductase from ‘Alphonso’ mango catalyzing biosynthesis of furaneol and reduction of reactive carbonyls. SpringerPlus, 2, 494.

    Article  Google Scholar 

  13. Chidley, H. G., Kulkarni, R. S., Pujari, K. H., Giri, A. P., & Gupta, V. S. (2013). Spatial and temporal changes in the volatile profile of Alphonso mango upon exogenous ethylene treatment. Food Chemistry, 136, 585–594.

    Article  CAS  Google Scholar 

  14. Yashoda, H. M., Prabha, T. N., & Tharanathan, R. N. (2007). Mango ripening—Role of carbohydrases in tissue softening. Food Chemistry, 102, 691–698.

    Article  CAS  Google Scholar 

  15. Frick, S., & Kutchan, T. M. (1999). Molecular cloning and functional expression of O-methyltransferase common to isoquinoline alkaloid and phenylpropanoid. The Plant Journal, 17, 329–339.

    Article  CAS  Google Scholar 

  16. Pandit, S. S., Kulkarni, R. S., Giri, A. P., Koellner, T. G., Degenhardt, J., Gershenzon, J., & Gupta, V. S. (2010). Expression profiling of various genes during the fruit development and ripening of mango. Plant Physiology and Biochemistry, 48, 426–433.

    Article  CAS  Google Scholar 

  17. Lovell, S. C., Davis, I. W., Adrendall, W. B., de Bakker, P. I. W., Word, J. M., Prisant, M. G., et al. (2003). Structure validation by C alpha geometry: Phi, psi and C beta deviation. Proteins-Structure Function and Genetics, 50, 437–450.

    Article  CAS  Google Scholar 

  18. Breathnach, R., & Chambon, P. (1981). Organization and expression of eukaryotic split genes-coding for proteins. Annual Review of Biochemistry, 50, 349–383.

    Article  CAS  Google Scholar 

  19. Zubieta, C., Kota, P., Ferrer, J. L., Dixon, R. A., & Noel, J. P. (2002). Structural basis for the modulation of lignin monomer methylation by caffeic acid/5-hydroxyferulic acid 3/5-O-methyltransferase. Plant Cell, 14, 1265–1277.

    Article  CAS  Google Scholar 

  20. Du, Q., Gong, C., Pan, W., & Zhang, D. (2013). Development and Application of Microsatellites in Candidate Genes Related to Wood Properties in the Chinese White Poplar (Populus tomentosa Carr.). DNA Research, 20, 31–44.

    Article  CAS  Google Scholar 

  21. Dunlevy, J. D., Soole, K. L., Perkins, M. V., Dennis, E. G., Keyzers, R. A., Kalua, C. M., & Boss, P. K. (2010). Two O-methyltransferases involved in the biosynthesis of methoxypyrazines: Grape-derived aroma compounds important to wine flavour. Plant Molecular Biology, 74, 77–89.

    Article  CAS  Google Scholar 

  22. Joshi, C. P., & Chiang, V. L. (1998). Conserved sequence motifs in plant S-adenosyl-l-methionine-dependent methyltransferases. Plant Molecular Biology, 37, 663–674.

    Article  CAS  Google Scholar 

  23. Li, L. G., Popko, J. L., Zhang, X. H., Osakabe, K., Tsai, C. J., Joshi, C. P., & Chiang, V. L. (1997). A novel multifunctional O-methyltransferase implicated in a dual methylation pathway associated with lignin biosynthesis in loblolly pine. Proceedings of the National Academy of Sciences of the United States of America, 94, 5461–5466.

    Article  CAS  Google Scholar 

  24. Tharanathan, R. N., Yashoda, H. M., & Prabha, T. N. (2006). Mango (Mangifera indica L.), The king of fruits—An overview. Food Reviews International, 22, 95–123.

    Article  CAS  Google Scholar 

  25. Singh, R., Gupta, P., Pandey, V. P., & Dwiyedi, U. N. (2011). Temporal expression of caffeic acid 3-O-methyltransferase (COMT) in mango (Mangifera indica var. Dashehari) fruit ripening. LWT-Food Science and Technology, 44, 1782–1785.

    Article  CAS  Google Scholar 

  26. Merchante, C., Vallarino, J. G., Osorio, S., Aragueez, I., Villarreal, N., Ariza, M. T., et al. (2013). Ethylene is involved in strawberry fruit ripening in an organ-specific manner. Journal of Experimental Botany, 64, 4421–4439.

    Article  CAS  Google Scholar 

  27. Cara, B., & Giovannoni, J. J. (2008). Molecular biology of ethylene during tomato fruit development and maturation. Plant Science, 175, 106–113.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

H.G.C., P.S.O. and A.B.D. thank CSIR for research fellowships. We are thankful to Dr. Balasaheb Sawant Konkan Agriculture University staff for the field support. A kind help by Prof. Sanjeev Galande, IISER, Pune, India to carry out real-time PCR is thankfully acknowledged. The work was funded by the Council of Scientific and Industrial Research, New Delhi, India under Project CSC0133 (FUN HEALTH) to CSIR-NCL.

Author information

Authors and Affiliations

  1. Plant Molecular Biology Unit, Division of Biochemical Sciences, CSIR-National Chemical Laboratory, Pune, 411008, India

    Hemangi G. Chidley, Pranjali S. Oak, Ashish B. Deshpande, Ashok P. Giri & Vidya S. Gupta

  2. Dr. Balasaheb Sawant Konkan Agriculture University, Dapoli, 415712, India

    Keshav H. Pujari

Authors
  1. Hemangi G. Chidley
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Pranjali S. Oak
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ashish B. Deshpande
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Keshav H. Pujari
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ashok P. Giri
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Vidya S. Gupta
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Vidya S. Gupta.

Ethics declarations

Conflict of interest

Provisional patent application related to this manuscript with the “Organization as applicant” and “Authors as inventors” has been filed with application number 1795/DEL/2015. No other competing interests exist.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Chidley, H.G., Oak, P.S., Deshpande, A.B. et al. Molecular Cloning and Characterization of O-Methyltransferase from Mango Fruit (Mangifera indica cv. Alphonso). Mol Biotechnol 58, 340–350 (2016). https://doi.org/10.1007/s12033-016-9933-2

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12033-016-9933-2

Keywords

Search

Navigation