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Reprogrammed Peripheral Blood Mononuclear Cells are Able to Survive Longer in Irradiated Female Mice

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Abstract

Induced multipotent stem (iMS) cells are originated from somatic cells and become multipotent by genetic and/or epigenetic modifications. Previous studies have shown that the fish oocytes extracts (FOE) can induce skin fibroblast cells into iMS cells. In this study, we aim to determine whether FOE can similarly induce mouse peripheral blood mononuclear cells (PBMCs) into the iMS state and if so, whether they can survive longer when they are transplanted into the irradiation female mice. PBMCs of GFP-transgenic male mice were cultured and transiently reprogrammed by FOE. They were deemed reaching the iMS state after detection of expression of stem cell markers. The iMS-like PBMCs were transplanted into female C57BL mice by tail vein injection. The spleen wet weights as well as numbers of colonies of the recipient mice were examined. The results showed the spleen wet weights and numbers of spleen colonies of FOE-induced group were all significantly higher than those of the non-induced group and negative control group. On day 90 after transplantation, FISH analysis detected the presence of Y chromosome in the induced group, but not of the other groups. The current findings demonstrate that FOE-induced PBMCs are able to survive longer in irradiated female mice.

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (31172170), 973 Projects (2012CB518106) and special funding by the China Postdoctoral Science Foundation (201104748).

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Authors and Affiliations

  1. Stem Cell Engineering Laboratory of Yunnan Province, Kunming General Hospital of Chengdu Military Command, Kunming, 650032, China

    Guang-Ping Ruan, Xiang-Qing Zhu, Xiang Yao, Rong-Qing Pang, Xue-Ming Cai, Jin-Xiang Wang, Jie He, Jing Zhao, Guang-Xu Zhu, Xin-Ming Xu & Xing-Hua Pan

  2. Shanghai First Maternity & Infants Hospital, Tongji University, Shanghai, 200040, China

    Yi-Bing Han

  3. Department of Dermatology, Second People’s Hospital of Yunnan Province, Kunming, 650021, China

    Guang-Hong Ruan

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  1. Guang-Ping Ruan
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Correspondence to Yi-Bing Han or Xing-Hua Pan.

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Ruan, GP., Han, YB., Ruan, GH. et al. Reprogrammed Peripheral Blood Mononuclear Cells are Able to Survive Longer in Irradiated Female Mice. Mol Biotechnol 55, 111–119 (2013). https://doi.org/10.1007/s12033-013-9661-9

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  • DOI: https://doi.org/10.1007/s12033-013-9661-9

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