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A Comparative Structural and Bioanalytical Study of IVIG Clinical Lots

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Abstract

Intravenous immunoglobulin are important bio-therapeutics used in the replacement therapy for primary and secondary immunodeficiencies, chronic inflammatory disorders and several autoimmune haematologic disorders. Currently, a number of immunoglobulin intravenous (IVIG) products have been approved by the Food and Drug Administration (FDA) and are available commercially. It is known that small differences in the manufacturing processes as well as in the formulations may affect their clinical efficacy and tolerability. Therefore, given the complexity of the multi-step process required for the isolation of IVIG from human plasma, it is necessary to ensure a rigorous quality control of final products. We show here that a set of different bioanalytical techniques can be conveniently used to comparatively characterize, at a quantitative and qualitative level, different lots of IVIG preparations and to unveil randomly occurring impurities which can also affect the overall product stability. We have used circular dichroism, surface plasmon resonance and two-dimensional electrophoresis (2DE), and have demonstrated that this combination of bioanalytical approaches is very useful to improve the quality control of antibodies and to monitor the reliability of the IVIG manufacturing process.

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Authors and Affiliations

  1. Istituto di Biostrutture e Bioimmagini, Consiglio Nazionale delle Ricerche, via Mezzocannone, 16, 80134, Naples, Italy

    Annamaria Sandomenico, Valeria Severino, Annalia Focà, Giuseppina Focà & Menotti Ruvo

  2. CIRPeB, via Mezzocannone, 16, 80134, Naples, Italy

    Annamaria Sandomenico, Annalia Focà, Giuseppina Focà & Menotti Ruvo

  3. Dipartimento di Scienze e Tecnologie Ambientali Biologiche e Farmaceutiche, via A. Vivaldi, 43, 81100, Caserta, Italy

    Valeria Severino & Angela Chambery

  4. Kedrion SpA, Loc. Ai Conti, 55051, Lucca, Castelvecchio Pascoli, Italy

    Claudio Farina

Authors
  1. Annamaria Sandomenico
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  2. Valeria Severino
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  3. Angela Chambery
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  4. Annalia Focà
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  5. Giuseppina Focà
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  6. Claudio Farina
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  7. Menotti Ruvo
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Corresponding authors

Correspondence to Annamaria Sandomenico or Menotti Ruvo.

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Sandomenico, A., Severino, V., Chambery, A. et al. A Comparative Structural and Bioanalytical Study of IVIG Clinical Lots. Mol Biotechnol 54, 983–995 (2013). https://doi.org/10.1007/s12033-013-9655-7

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  • DOI: https://doi.org/10.1007/s12033-013-9655-7

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