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Analysis of Product Quality of Complex Polymeric IgM Produced by CHO Cells

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Animal Cell Biotechnology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2095))

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Abstract

Immunoglobulin M (IgM) antibodies are considered as promising biopharmaceutical drugs in the future despite recombinant production is quite challenging as incomplete polymer formation or nucleic acid adherence can decrease the quality of the IgM preparation. Therefore, we defined densitometric and chromatographic methods as suitable tools to analyze the polymer distribution and the remaining nucleic acid content after initial IgM purification. Additionally, the quality of the glycosylation pattern is an important parameter for biological safety and efficacy.

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Author information

Authors and Affiliations

  1. Department of Biotechnology, VIBT, University of Natural Resources and Life Sciences (BOKU), Vienna, Austria

    Julia Hennicke & Renate Kunert

Authors
  1. Julia Hennicke
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  2. Renate Kunert
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Corresponding author

Correspondence to Renate Kunert .

Editor information

Editors and Affiliations

  1. Institute of Bioprocess and Biosystems Engineering, Hamburg University of Technology (TUHH), Hamburg, Germany

    Ralf Pörtner

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Hennicke, J., Kunert, R. (2020). Analysis of Product Quality of Complex Polymeric IgM Produced by CHO Cells. In: Pörtner, R. (eds) Animal Cell Biotechnology. Methods in Molecular Biology, vol 2095. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0191-4_17

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  • DOI: https://doi.org/10.1007/978-1-0716-0191-4_17

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0190-7

  • Online ISBN: 978-1-0716-0191-4

  • eBook Packages: Springer Protocols

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