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Genetic Diversity Analysis in Piper Species (Piperaceae) Using RAPD Markers

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Abstract

The genetic diversity of eight species of Piper (Piperaceae) viz., P. nigrum, P. longum, P. betle, P. chaba, P. argyrophyllum, P. trichostachyon, P. galeatum, and P. hymenophyllum from Kerala state, India were analyzed by Random amplified polymorphic DNA (RAPD). Out of 22 10-mer RAPD primers screened, 11 were selected for comparative analysis of different species of Piper. High genetic variations were found among different Piper species studied. Among the total of 149 RAPD fragments amplified, 12 bands (8.05%) were found monomorphic in eight species. The remaining 137 fragments were found polymorphic (91.95%). Species-specific bands were found in all eight species studied. The average gene diversity or heterozygosity (H) was 0.33 across all the species, genetic distances ranged from 0.21 to 0.69. The results of this study will facilitate germplasm identification, management, and conservation.

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Acknowledgments

The authors are grateful to Rev. Dr. Abraham Mulamoottil, Principal; Dr. C. Balagopalan, Dean, School of Biosciences; and Dr. P. K. Shaji, Prof. Phytomedical Science and Technology, MACFAST for encouragement, support, and guidance. The authors are also gratefully acknowledged to Dr. A. Gopalakrishnan, Principal Scientist, NBFGR Cochin Unit, CMFRI for providing the excellent facilities for gel documentation. We also thank to Dr. Anathbandhu Chaudhuri, Dr. Adam Szlachetka, University of Nebraksa Medical Center, Omaha, USA and Dr. G. Ravikanth, Conservation Genetics Lab, ATREE, Bangalore for critical reading and comments on the manuscript.

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Authors and Affiliations

  1. Molecular Biology and Genetic Engineering Research Unit (MBGERU), School of Biosciences, Mar Athanasios College for Advanced Studies, Tiruvalla (MACFAST), Kerala, 689101, India

    Sandeep Sen, Reby Skaria & P. M. Abdul Muneer

  2. Department of Pharmacology and Experimental Neuroscience, The Center for Neurovirology and Neurodegenerative Disorders, University of Nebraska Medical Center, Omaha, NE, 68198, USA

    P. M. Abdul Muneer

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  1. Sandeep Sen
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Correspondence to P. M. Abdul Muneer.

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Sen, S., Skaria, R. & Muneer, P.M.A. Genetic Diversity Analysis in Piper Species (Piperaceae) Using RAPD Markers. Mol Biotechnol 46, 72–79 (2010). https://doi.org/10.1007/s12033-010-9281-6

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