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No association between XRCC3 Thr241Met and XPD Lys751Gln polymorphisms and the risk of colorectal cancer in West Algerian population: a case–control study

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Abstract

Colorectal cancer (CRC) is a complex and multifactorial disease, in which genetic and environmental factors both seem to play a part. Many epidemiological studies have explored the association between genetic polymorphisms of X-ray repair cross-complementing group 3 (XRCC3) (Thr241Met) and Xeroderma pigmentosum group D (XPD) lysine to glutamine at codon 751 (Lys751Gln) and risk of CRC in various populations; however, the results are controversial. We conducted this case–control study in a West Algerian population to assess the potential role of this genetic polymorphism on the risk of CRC in this population. Genomic DNA was extracted from blood samples collected from 129 sporadic CRC patients and 148 normal controls. The polymorphisms were determined by pyrosequencing technique. The distribution of XRCC3 Thr241Met and XPD Lys751Gln genotypes among controls did not differ significantly from those predicted by the Hardy–Weinberg distribution (p > 0.05). There were no significant differences in the genotypes distribution and allele frequencies between CRC patients and controls. A significant association was found between the combined heterozygous of XRCC3 and homozygous variant of XPD gene and CRC. This is the first study on DNA repair genetic polymorphisms in West Algerian population, and it suggests that the XRCC3 Thr241Met and XPD Lys751Gln polymorphisms may not be associated with the CRC risk in this population.

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Acknowledgments

The authors of this article are grateful to all subjects for donating their blood for this study. In addition, they thank all the collaborators for collection of blood samples and information.

Conflict of interests

The authors have declared that no conflict of interests exists.

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Authors and Affiliations

  1. Laboratoire de Génétique Moléculaire et Cellulaire, Département de Génétique Moléculaire Appliquée, Faculté des sciences de la nature et de la vie, Université des Sciences et de la Technologie d’Oran–Mohamed BOUDIAF-USTOMB, BP 1505, El M’naouer, 31000, Oran, Algeria

    Fatima Zohra Moghtit, Meriem Samia Aberkane, Lotfi Louhibi, Mostefa Fodil, Sounnia Mediene-Benchekor, Faouzia Zemani-Fodil, Abdallah Boudjema & Nadhira Saidi-Mehtar

  2. Département de Pharmacie, Faculté de Médecine, Université d’Oran, BP 1524, El M’naouer, 31000, Oran, Algeria

    Meriem Samia Aberkane

  3. Laboratoire de Pharmacologie des Agents anticancéreux, INSERM U916, Institut Bergonié, 229 cours de l’Argonne, 33076, Bordeaux cedex, France

    Valérie Le Morvan, Ricardo Bellot & Jacques Robert

  4. Centre Anti-Cancer (Emir Abdelkader), Route de Misserghine, 31000, Oran, Algeria

    Abdelkader Bousahba & Ahlem Megaiz

  5. Département de Biotechnologie, Faculté des Sciences de la Nature et de la Vie, Université d’Oran, BP 1524, El M’naouer, 31000, Oran, Algeria

    Mostefa Fodil & Sounnia Mediene-Benchekor

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  1. Fatima Zohra Moghtit
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  2. Meriem Samia Aberkane
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  3. Valérie Le Morvan
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Correspondence to Fatima Zohra Moghtit.

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Moghtit, F.Z., Aberkane, M.S., Le Morvan, V. et al. No association between XRCC3 Thr241Met and XPD Lys751Gln polymorphisms and the risk of colorectal cancer in West Algerian population: a case–control study. Med Oncol 31, 942 (2014). https://doi.org/10.1007/s12032-014-0942-3

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