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Polymorphism in pre-miR-30c contributes to gastric cancer risk in a Chinese population

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Abstract

To explore the association between single nucleotide polymorphisms (SNPs) A/G in pre-miR-30c and gastric cancer risk and to assess various genotypes that affected the survival of patients, we performed a hospital-based, case–control study in Inner Mongolia autonomous region of China. A total of 240 gastric cancer patients and 240 age- and sex-matched cancer-free controls collected from 2006 to 2011 were enrolled in this study. Polymorphism rs928508 in pre-miR-30c was analyzed by TaqMan SNP genotyping assay. The expression of mature miR-30c was detected by real-time quantitative reverse transcription PCR in 48 gastric cancer specimens. Survival analysis was analyzed by the Kaplan–Meier survival curves. The genotype frequencies of pre-miR-30c A/G in gastric cancer patients were obviously different from those in the controls (P = 0.022). AA genotype carriers were associated with an increased risk of gastric cancer compared with GG genotype (adjusted odds ratio (OR) = 1.83, 95% confidence interval (CI): 1.07–3.15, P = 0.029). Moreover, the gastric cancer risk especially elevated in older individuals (aged >60 years), males, nonsmokers, and Helicobacter pylori (H. pylori)-infected individuals (adjusted OR = 2.66, 95% CI: 1.38–5.13, P = 0.004; adjusted OR = 1.90, 95% CI: 1.10–3.27, P = 0.022; adjusted OR = 1.94, 95% CI: 1.12–3.35, P = 0.018; adjusted OR = 1.83, 95% CI: 1.08–3.10, P = 0.024, respectively). Further stratified analysis indicated that AA genotype facilitated developing of gastric cancer with lymph node metastasis (adjusted OR = 2.23, 95% CI: 1.07–4.64, P = 0.032). Expression analysis detected that rs928508 AA showed a significantly increased level of mature miR-30c compared with GG or AG/GG genotype (P = 0.011 or P = 0.013). Patients with AA genotype were associated with unfavorable outcome in overall survival compared with AG/GG genotype (Log rank 5.848, P = 0.016). This study demonstrates that pre-miR-30c A/G polymorphism may be associated with an increased risk of gastric cancer in a Chinese population through altering mature miR-30c expression. However, their role as novel biomarkers for the diagnosis and prognosis of gastric diseases still needs to be systematically evaluated.

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Acknowledgments

The authors thank Jia SQ for her collected samples from the First Affiliated Hospital of Inner Mongolia Medical College. This study was supported by a grant for scientific research from the Affiliated Hospital of Inner Mongolia Medical College NYFZD 2010-0008.

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Authors and Affiliations

  1. Department of Cell Biology, Capital Medical University, Beijing, 100069, China

    Yong-ping Mu & Xiu-lan Su

  2. Clinical Medical Research Center of the Affiliated Hospital, Inner Mongolia Medical College, 1 Tongdao North Street, Hohhot, 010050, China

    Xiu-lan Su

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  1. Yong-ping Mu
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Mu, Yp., Su, Xl. Polymorphism in pre-miR-30c contributes to gastric cancer risk in a Chinese population. Med Oncol 29, 1723–1732 (2012). https://doi.org/10.1007/s12032-011-0115-6

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  • DOI: https://doi.org/10.1007/s12032-011-0115-6

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