Abstract
Exosomes are cell-derived membrane vesicles with cargo that can be transported into receiver cells to exert their biological roles. Exosomal RNA signature profiles and exosome-derived proteomics are often used to explore the molecular regulation of diseases, and can mirror the conditional state of their tissue of origin, thus serving as biomarkers. The onset of meningeal carcinomatosis (MC) is concealed, and early diagnosis is difficult. To enable early diagnosis of MC, it is essential to identify new biomarkers. Few studies have investigated the function of exosomes in MC. In this study, high-throughput sequencing was used to examine the mRNA profiles of exosomes in the cerebrospinal fluid (CSF) of patients with MC. We further analyzed the functions and signaling pathways associated with the differentially expressed genes in exosomes to reveal the putative mechanisms by which the exosomal mRNAs function in MC. In summary, this study identified biomarker candidates for MC, and provided new insights into the significant role of exosomal mRNA regulation in MC.
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Funding
The study was supported by the fund of the National Natural Science Foundation of China (81571052, 81973630), Medical Science and Technology Development Plan of Shandong Province (2014WS0158). We thank Cloud-Seq Biotech Ltd. Co. (Shanghai, China) for the whole transcriptome sequencing service and the subsequent bioinformatics analysis.
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Cheng, P., Feng, F., Yang, H. et al. Detection and significance of exosomal mRNA expression profiles in the cerebrospinal fluid of patients with meningeal carcinomatosis. J Mol Neurosci 71, 790–803 (2021). https://doi.org/10.1007/s12031-020-01701-w
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DOI: https://doi.org/10.1007/s12031-020-01701-w