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Unrestricted Somatic Stem Cells Loaded in Nanofibrous Conduit as Potential Candidate for Sciatic Nerve Regeneration

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Abstract

Motor and sensory recovery following critical size peripheral nerve defects is often incomplete. Although nerve grafting has been proposed as the gold standard, it is associated with several disadvantages. Here we report a novel approach to peripheral nerve repair using Human Unrestricted Somatic Stem Cells (USSC) delivered through an electrospun neural guidance conduit. Conduits were produced from PCL and gelatin blend. Several in vitro methods were utilized to investigate the conduit’s physicochemical and biological characteristics. Nerve regeneration was studied across a 10-mm sciatic nerve gap in Wistar rats. For functional analysis, the conduits were seeded with 3 × 104 USSCs and implanted into a 10-mm sciatic nerve defect. After 14 weeks, the results of functional recovery analysis and histopathological examinations showed that animals implanted with USSC containing conduits exhibited improved functional and histopathological recovery which was more close to the autograft group compared to other groups. Our results support the potential applicability of USSCs to treat peripheral nerve injury in the clinic.

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Acknowledgment

The present study was supported by Shahroud University of medical sciences. We hereby acknowledge the research deputy for grant No 97130138.

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  1. Saeed Farzamfar, Arian Ehterami and Majid Salehi contributed equally to this work.

Authors and Affiliations

  1. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

    Saeed Farzamfar & Ahmad Vaeez

  2. Department of Mechanical and Aerospace Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Arian Ehterami

  3. Department of Tissue Engineering, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran

    Majid Salehi

  4. Tissue Engineering and Stem Cells Research Center, Shahroud University of Medical Sciences, Shahroud, Iran

    Majid Salehi & Amir Atashi

  5. Department of Hematology, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran

    Amir Atashi

  6. Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Hamed Sahrapeyma

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  1. Saeed Farzamfar
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Farzamfar, S., Ehterami, A., Salehi, M. et al. Unrestricted Somatic Stem Cells Loaded in Nanofibrous Conduit as Potential Candidate for Sciatic Nerve Regeneration. J Mol Neurosci 67, 48–61 (2019). https://doi.org/10.1007/s12031-018-1209-9

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