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The Expression Changes of Myelin and Lymphocyte Protein (MAL) Following Optic Nerve Crush in Adult Rats Retinal Ganglion Cells

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Abstract

Myelin and lymphocyte protein (MAL), a component of compact myelin, is highly expressed in oligodendrocytes and Schwann cells. It has been reported that MAL may play a vital role in the process of neuronal apoptosis following acute spinal cord injury. However, acquaintance regarding its distribution and possible function in the retina is limited. Therefore, in a rodent model of optic nerve crush (ONC), the dynamic changes of MAL in retina was detected. The expression of MAL was mainly located in the retinal ganglion cells (RGCs) and was increased strongly after ONC. The peak of MAL expression appeared on the third day. In addition, there was a concomitant upregulation of active-caspase-3, which also co-localized with MAL in RGCs. Moreover, co-localization of MAL with terminal deoxynucleotidyl transferase-mediated biotinylated-dUTP nick-end labeling (TUNEL) was detected in RGCs after ONC. Collectively, all these results suggested that the upregulation of MAL might play an important role in the pathophysiology of RGCs after ONC.

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Acknowledgments

This work was supported by the Specialized Research Foundation for Doctoral Program of Higher Education of China (No. 20120171110086) and Science and Technology Planning Project of Guangzhou China (No. 11C22060787).

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Authors and Affiliations

  1. State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, Guangdong Province, People’s Republic of China

    Yongsheng Huang, Yue Xu, Qiaochu Cheng, Shanshan Yu, Yi Gao, Yuan Sun, Jiawei Wang, Fan Xu & Xiaoling Liang

  2. Department of Ophthalmology, Eye and ENT Hospital of Fudan University, Shanghai, People’s Republic of China

    Qinmeng Shu

  3. Department of Ophthalmology, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong Province, People’s Republic of China

    Cheng Yang

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  1. Yongsheng Huang
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  2. Yue Xu
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Correspondence to Xiaoling Liang.

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Yongsheng Huang and Yue Xu contributed equally to this work

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Huang, Y., Xu, Y., Cheng, Q. et al. The Expression Changes of Myelin and Lymphocyte Protein (MAL) Following Optic Nerve Crush in Adult Rats Retinal Ganglion Cells. J Mol Neurosci 54, 614–621 (2014). https://doi.org/10.1007/s12031-014-0332-5

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