Journal of Molecular Neuroscience

, Volume 54, Issue 2, pp 156–163 | Cite as

Glial Cell-Derived Neurotrophic Factor Attenuates Neuropathic Pain in a Mouse Model of Chronic Constriction Injury: Possible Involvement of E-cadherin/p120ctn Signaling

  • Cunjin Wang
  • Hongjun Wang
  • Jun Pang
  • Li Li
  • Suming Zhang
  • Ge Song
  • Na Li
  • Junping Cao
  • Licai Zhang
Article

Abstract

Treating neuropathic pain is a major clinical challenge, and several key molecules associated with nociception have been suggested as potential targets for novel analgesics. Many studies have reported the anti-nociceptive effects of glial cell-derived neurotrophic factor (GDNF), but the underlying mechanism remains largely unknown. The present study was performed to assess the effects of GDNF in a mouse model of chronic constriction injury (CCI)-induced neuropathic pain. We also determined the potential role of E-cadherin/p120 catenin (p120ctn) signaling in these effects. Mice received an intrathecal acute injection of PBS, GDNF, and DECMA-1 (an E-cadherin functional blocking antibody) or a combination of DECMA-1 with GDNF on the testing days. Our results demonstrated that CCI caused a rapid decrease in E-cadherin and membrane-associated p120ctn in the spinal dorsal horn. Together, these data demonstrated that E-cadherin-associated p120ctn was upregulated by GDNF and that this upregulation was inhibited by pre-treatment with DECMA-1. Moreover, DECMA-1 significantly inhibited the effect of GDNF on thermal hyperalgesia. These data suggest that GDNF might have a therapeutic potential for the treatment of CCI-induced neuropathic pain and that the E-cadherin/p120ctn might play a role in GDNF-induced attenuation of thermal hyperalgesia.

Keywords

Glial cell-derived neurotrophic factor E-cadherin p120 catenin Neuropathic pain Chronic constrictive injury Spinal cord 

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Cunjin Wang
    • 1
    • 2
  • Hongjun Wang
    • 1
    • 2
  • Jun Pang
    • 1
    • 2
  • Li Li
    • 1
    • 2
  • Suming Zhang
    • 1
    • 2
  • Ge Song
    • 1
    • 2
  • Na Li
    • 1
    • 2
  • Junping Cao
    • 1
    • 2
  • Licai Zhang
    • 1
    • 2
  1. 1.Jiangsu Province Key Laboratory of AnesthesiologyXuzhou Medical CollegeXuzhouChina
  2. 2.Jiangsu Province Key Laboratory of Anesthesia and Analgesia Application TechnologyXuzhou Medical CollegeXuzhouChina

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