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Protection Effect of GDNF and Neurturin on Photosensitized Crayfish Neurons and Glial Cells

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Abstract

Neurons and glial cells can protect each other from stress and following death by mutual exchange with neurotrophins. In order to examine involvement of different neurotrophic factors in neuroglial interactions in a photosensitized crayfish stretch receptor, a simple model object consisting of only two sensory neurons enveloped by glial cells, we studied the influence of glial cell line-derived neurotrophic factor (GDNF), neurturin, and ciliary neurotrophic factor (CNTF) on its photodynamic injury. Photodynamic treatment, which causes strong oxidative stress, induced firing abolition and necrosis of neurons, necrosis, and apoptosis of glial cells. GDNF significantly reduced photoinduced neuronal necrosis and neurturin but not CNTF showed a similar tendency. Both of them significantly reduced necrosis and apoptosis of glial cells. At the ultrastructural level, neurons and glial cells treated with GDNF in the darkness contained large mitochondria with well-developed cristae, numerous ribosomes, polysomes, rough endoplasmic reticulum (ER), and dictyosomes. This indicated the high level of bioenergetic, biosynthetic, and transport processes. Photodynamic treatment caused swelling and vacuolization of mitochondria, dictyosomes, and ER. It also impaired formation of glial protrusions and double membrane vesicles that transfer glial material into the neuron. GDNF prevented photoinduced mitochondria swelling that disturbed the cellular bioenergetics and cytoplasm vacuolization associated with injury of intracellular organelles. It also preserved the structures involved in protein synthesis and transport: rough ER, dictyosomes, polysomes, microtubule bundles, submembrane cisterns, and double membrane vesicles. GDNF-mediated maintenance of metabolism and ultrastructure of photosensitized neurons and glial cells may be the basis of its neuro- and glia protective effects.

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Acknowledgments

The work was supported by grants from the Ministry of Education and Science of Russian Federation (grants 2.1.1/6185 and 1.16.11) and the Russian Foundation for Basic Research (grants 08-04-01322 and 11-04-01476).

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Authors and Affiliations

  1. Department of Biophysics and Biocybernetics, Institute of Neurocybernetics, Southern Federal University, 194/1 Stachky Ave., NII NK, Rostov-on-Don, 344090, Russia

    Anatoly Uzdensky, Maxim Komandirov, Grigory Fedorenko & Andrej Lobanov

  2. Southern Scientific Center RAN, Rostov-on-Don, Russia

    Grigory Fedorenko

Authors
  1. Anatoly Uzdensky
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  2. Maxim Komandirov
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  3. Grigory Fedorenko
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  4. Andrej Lobanov
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Correspondence to Anatoly Uzdensky.

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Uzdensky, A., Komandirov, M., Fedorenko, G. et al. Protection Effect of GDNF and Neurturin on Photosensitized Crayfish Neurons and Glial Cells. J Mol Neurosci 49, 480–490 (2013). https://doi.org/10.1007/s12031-012-9858-6

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  • DOI: https://doi.org/10.1007/s12031-012-9858-6

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