Abstract
Endoplasmic reticulum (ER) stress may play a role in status epilepticus (SE). Sprague–Dawley rats were randomized into three groups: control (saline), SE (pentylenetetrazol), and dentate gyrus (DG), pretreated with 2-deoxy-d-glucose (2-DG). Expression levels of glucose-regulated protein 78 (GRP78), CCAAT/enhancer-binding protein homologous protein (CHOP), eukaryotic initiation factor 2α (eIF2α), and protein kinase RNA-like ER kinase (PERK) were determined. CHOP messenger RNA (mRNA) and protein expression levels in SE group were significantly increased as compared to the control group (P < 0.0001), and significantly decreased in DG group as compared to the SE group (P < 0.0001). Phosphorylated eIF-2α protein expression level in SE group was significantly increased as compared to the control group (P < 0.0001) and significantly decreased in DG group as compared to the SE group (P < 0.0001). GRP messenger RNA expression levels and protein levels in SE group was significantly increased as compared to the control group (P < 0.0001) and significantly decreased in DG group as compared to the SE group (P < 0.0001). Phosphorylated PERK protein expression level in SE group was significantly increased as compared to the control group (P < 0.0001), and significantly decreased in DG group as compared to the SE group (P < 0.0001) at the time of 12 and 24 h. Our results suggest that brain injury from SE might involve ER stress via the pro-apoptotic PERK–eIF2α–CHOP signaling pathway.
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References
Brennan GP, Jimenez-Mateos EM, McKiernan RC, Engel T, Tzivion G, Henshall DC (2013) Transgenic overexpression of 14-3-3 zeta protects hippocampus against endoplasmic reticulum stress and status epilepticus in vivo. PLoS One 8:e54491
Chen J, Qin J, Liu X et al (2008) Nitric oxide-mediated neuronal apoptosis in rats with recurrent febrile seizures through endoplasmic reticulum stress pathway. Neurosci Lett 443:134–139
Engel T, Sanz-Rodgriguez A, Jimenez-Mateos EM et al (2013) CHOP regulates the p53-MDM2 axis and is required for neuronal survival after seizures. Brain 136:577–592
Henshall DC, Murphy BM (2008) Modulators of neuronal cell death in epilepsy. Curr Opin Pharmacol 8:75–81
Lado FA, Sperber EF, Moshe SL (2001) Anticonvulsant efficacy of gabapentin on kindling in the immature brain. Epilepsia 42:458–463
Little E, Tocco G, Baudry M, Lee AS, Schreiber SS (1996) Induction of glucose-regulated protein (glucose-regulated protein 78/BiP and glucose-regulated protein 94) and heat shock protein 70 transcripts in the immature rat brain following status epilepticus. Neuroscience 75:209–219
Liu GL, Wang KY, Guo H, Zhao SJ, Shen Y, Zhao YB (2013) Inositol-requiring protein 1alpha signaling pathway is activated in the temporal cortex of patients with mesial temporal lobe epilepsy. Neurol Sci 34:357–364
Paschen W (2004) Endoplasmic reticulum dysfunction in brain pathology: critical role of protein synthesis. Curr Neurovasc Res 1:173–181
Sokka AL, Putkonen N, Mudo G et al (2007) Endoplasmic reticulum stress inhibition protects against excitotoxic neuronal injury in the rat brain. J Neurosci 27:901–908
Stafstrom CE, Roopra A, Sutula TP (2008) Seizure suppression via glycolysis inhibition with 2-deoxy-d-glucose (2DG). Epilepsia 49(Suppl 8):97–100
Stafstrom CE, Ockuly JC, Murphree L, Valley MT, Roopra A, Sutula TP (2009) Anticonvulsant and antiepileptic actions of 2-deoxy-d-glucose in epilepsy models. Ann Neurol 65:435–447
Torres-Peraza JF, Engel T, Martin-Ibanez R et al (2013) Protective neuronal induction of ATF5 in endoplasmic reticulum stress induced by status epilepticus. Brain 136:1161–1176
Yamamoto A, Murphy N, Schindler CK et al (2006) Endoplasmic reticulum stress and apoptosis signaling in human temporal lobe epilepsy. J Neuropathol Exp Neurol 65:217–225
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This study was supported by Key Project supported by Medical Science and Technology Development Foundation, Nanjing Department of Health (ZKX10024).
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Chen, J., Zheng, G., Guo, H. et al. Role of Endoplasmic Reticulum Stress via the PERK Signaling Pathway in Brain Injury from Status Epilepticus. J Mol Neurosci 53, 677–683 (2014). https://doi.org/10.1007/s12031-014-0236-4
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DOI: https://doi.org/10.1007/s12031-014-0236-4