Abstract
P-glyprotein (multidrug resistance gene 1, MDR1) was the first isolated and most extensively investigated refractory epilepsy-related drug-resistant protein. Coriaria lactone (CL) can induce P-glyprotein expression of brain capillary and astrocytes in vivo. We established the primary rat brain microvascular endothelial cell (BMECs) models overexpressing P-glycoprotein induced by CL successfully and infected them with adenovirus vector, which carry small interfering RNA (siRNA) designed to target MDR1b. MDR1b mRNA levels and P-glyprotein expression in experimental group decreased significantly 72 h after infection, and the fluorescence intensity of rhodamine within the cells of experimental group increased significantly. So, CL can induce P-glyprotein overexpression in BMECs in vitro, and siRNA can effectively inhibit expression of P-glyprotein and decrease its “pumping out” function. This may offer a new method and rationale for studying the mechanism of refractory epilepsy and relevant gene therapy.
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This work was supported by the National Natural Science Foundation of China and West China Hospital (nos. 30770749/C030307, 30370513/C030307).
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Tian, L., Chen, L., Yang, T. et al. RNAi Inhibits Coriaria Lactone-Induced MDR1b Overexpression in Rat Brain Microvascular Endothelial Cells. J Mol Neurosci 39, 284–293 (2009). https://doi.org/10.1007/s12031-009-9198-3
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DOI: https://doi.org/10.1007/s12031-009-9198-3