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Frequency of Hepatitis C Virus Genotypes in Patients Who Had Hepatocellular Carcinoma in Gastroenterological Hospitals of Tabriz

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Abstract

Background

Generally, hepatitis C has been identified as one of the major health issues that about 3% of the world’s population have been threatened and affected by it (about 170 million people), and also, it can be considered a factor in acute and chronic hepatitis.

Methods

The aim of this study is to determine the prevalence of HCV genotypes in Azerbaijan patients. In this study, sampling was done on the referred patients to the hospitals (Mahallati and Behbud Hospital). RNA was extracted after isolation of plasma, and then, after the synthesizing of cDNA, the sample was carried out to the laboratory for performing the real-time PCR in order to determine the genotypes.

Results

The evaluation of HCV genotypes in positive plasma samples showed that dominant subsets were remarkable and the mean age of the patients was 37/3 ± 11/8 (in the age range of 2–63). Among the 235 patients,139 of them (59%) were male. Statistically, the average number of women was more than men (T test, P < 0/05). 1b genotype was reported 70% in the patients above 40 years old, and also, it was reported as 71/6% in the patients under 40 years old that was not statistically significant. The incidence of serotype 3a was higher among the patients younger than 40 years old (3a was 18.1% vs. 15%), and this serotype was prevalent among men (3a was 18.7% vs. 14.6%), which was statistically significant.

Conclusion

The findings indicate that among Azerbaijan’s patients with chronic hepatitis C, genotypes 1b (71.1%) and 3a (17%) were dominant.

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References

  1. Rho J, et al. Hepatitis C virus (HCV) genotyping by annealing reverse transcription-PCR products with genotype-specific capture probes. J Microbiol. 2008;46(1):81–7.

    Article  CAS  Google Scholar 

  2. Ly KN, et al. The increasing burden of mortality from viral hepatitis in the United States between 1999 and 2007. Ann Intern Med. 2012;156(4):271–8.

    Article  Google Scholar 

  3. Supram HS, Gokhale S, Sathian B, Bhatta DR. Hepatitis B virus (HBV) and hepatitis C virus (HCV) co-infection among HIV infected individuals at tertiary care hospital in Western Nepal. Nepal J Epidemiol. 2015;5(2):488–93.

    Article  Google Scholar 

  4. Messina JP, Humphreys I, Flaxman A, Brown A, Cooke GS, Pybus OG, et al. Global distribution and prevalence of hepatitis C virus genotypes. Hepatology. 2015;61(1):77–87.

    Article  Google Scholar 

  5. Young AM, Crosby RA, Oser CB, Leukefeld CG, Stephens DB, Havens JR. Hepatitis C viremia and genotype distribution among a sample of nonmedical prescription drug users exposed to HCV in rural Appalachia. J Med Virol. 2012;84(9):1376–87.

    Article  Google Scholar 

  6. Smith DB, Bukh J, Kuiken C, Muerhoff AS, Rice CM, Stapleton JT, et al. Expanded classification of hepatitis C virus into 7 genotypes and 67 subtypes: updated criteria and genotype assignment web resource. Hepatology. 2014;59(1):318–27.

    Article  Google Scholar 

  7. Zarkesh-Esfahani SH, Kardi MT, Edalati M. Hepatitis C virus genotype frequency in Isfahan province of Iran: a descriptive cross-sectional study. Virol J. 2010;7:69.

    Article  Google Scholar 

  8. Umar M, Bushra HT, Ahmad M, Data A, Ahmad M, Khurram M, et al. Hepatitis C in Pakistan: a review of available data. Hepat Mon. 2010;10(3):205–14.

    PubMed  PubMed Central  Google Scholar 

  9. Dusheiko G, Schmilovitz-Weiss H, Brown D, McOmish F, Yap PL, Sherlock S, et al. Hepatitis C virus genotypes: an investigation of type-specific differences in geographic origin and disease. Hepatology. 1994;19(1):13–8.

    Article  CAS  Google Scholar 

  10. Chlabicz S, Flisiak R, Lapinski TW, Kowalczuk O, Wiercinska-Drapalo A, Pytel-Krolczuk B, et al. Epidemiological features of patients infected with HCV genotype 4 in Poland: epidemiology of HCV genotype 4 in Poland. Hepat Mon. 2011;11(3):191–4.

    PubMed  PubMed Central  Google Scholar 

  11. Zein NN, Rakela J, Krawitt EL, Reddy KR, Tominaga T, Persing DH. Hepatitis C virus genotypes in the United States: epidemiology, pathogenicity, and response to interferon therapy. Ann Intern Med. 1996;125(8):634–9.

    Article  CAS  Google Scholar 

  12. Fallahian F, Najafi A. Epidemiology of hepatitis C in the Middle East. Saudi J Kidney Dis Transpl. 2011;22(1):1–9.

    PubMed  Google Scholar 

  13. Bokharaei-Salim F, et al. Distribution of hepatitis C virus genotypes among azerbaijani patients in capital city of Iran-Tehran. Hepat Mon. 2013;13(9):e13699.

    Article  Google Scholar 

  14. Samimi-Rad K, et al. Molecular epidemiology of hepatitis C virus in Iran as reflected by phylogenetic analysis of the NS5B region. J Med Virol. 2004;74(2):246–52.

    Article  CAS  Google Scholar 

  15. Abdel-Moneim AS, Bamaga MS, Shehab GM, Abu-Elsaad AA, Farahat FM. HCV infection among Saudi population: high prevalence of genotype 4 and increased viral clearance rate. PLoS One. 2012;7(1):e29781.

    Article  CAS  Google Scholar 

  16. Altuglu I, Soyler I, Ozacar T, Erensoy S. Distribution of hepatitis C virus genotypes in patients with chronic hepatitis C infection in Western Turkey. Int J Infect Dis. 2008;12(3):239–44.

    Article  CAS  Google Scholar 

  17. Cornberg M, et al. A systematic review of hepatitis C virus epidemiology in Europe, Canada and Israel. Liver Int. 2011;31(Suppl 2):30–60.

    Article  Google Scholar 

  18. Ramia S, Eid-Fares J. Distribution of hepatitis C virus genotypes in the Middle East. Int J Infect Dis. 2006;10(4):272–7.

    Article  CAS  Google Scholar 

  19. Viazov S, Kuzin S, Paladi N, Tchernovetsky M, Isaeva E, Mazhul L, et al. Hepatitis C virus genotypes in different regions of the former Soviet Union (Russia, Belarus, Moldova, and Uzbekistan). J Med Virol. 1997;53(1):36–40.

    Article  CAS  Google Scholar 

  20. Olinger CM, Lazouskaya NV, Eremin VF, Muller CP. Multiple genotypes and subtypes of hepatitis B and C viruses in Belarus: similarities with Russia and western European influences. Clin Microbiol Infect. 2008;14(6):575–81.

    Article  CAS  Google Scholar 

  21. Tarantola A, Abiteboul D, Rachline A. Infection risks following accidental exposure to blood or body fluids in health care workers: a review of pathogens transmitted in published cases. Am J Infect Control. 2006;34(6):367–75.

    Article  Google Scholar 

  22. Kurbanov F, Tanaka Y, Sugauchi F, Kato H, Ruzibakiev R, Zalyalieva M, et al. Hepatitis C virus molecular epidemiology in Uzbekistan. J Med Virol. 2003;69(3):367–75.

    Article  CAS  Google Scholar 

  23. Khan A, et al. Epidemiological and clinical evaluation of hepatitis B, hepatitis C, and delta hepatitis viruses in Tajikistan. J Med Virol. 2008;80(2):268–76.

    Article  CAS  Google Scholar 

  24. Bdour S. Hepatitis C virus infection in Jordanian haemodialysis units: serological diagnosis and genotyping. J Med Microbiol. 2002;51(8):700–4.

    Article  Google Scholar 

  25. Ray SC, et al. Genetic epidemiology of hepatitis C virus throughout Egypt. J Infect Dis. 2000;182(3):698–707.

    Article  CAS  Google Scholar 

  26. Sharvadze L, et al. Prevalence of HCV and genotypes distribution in general population of Georgia. Georgian Med News. 2008;165:71–7.

    Google Scholar 

  27. Alfaresi MS. Prevalence of hepatitis C virus (HCV) genotypes among positive UAE patients. Mol Biol Rep. 2011;38(4):2719–22.

    Article  CAS  Google Scholar 

  28. Keyvani H, Alizadeh AH, Alavian SM, Ranjbar M, Hatami S. Distribution frequency of hepatitis C virus genotypes in 2231 patients in Iran. Hepatol Res. 2007;37(2):101–3.

    Article  Google Scholar 

  29. Daw MA, Dau AA. Hepatitis C virus in Arab world: a state of concern. ScientificWorldJournal. 2012;2012:719494.

    PubMed  PubMed Central  Google Scholar 

  30. S. Pacsa, A., et al., Genotypes of hepatitis C virus in Kuwait. Vol. 10. 2001. 55–57.

  31. Shobokshi OA, Serebour FE, Skakni LI. Hepatitis C genotypes/subtypes among chronic hepatitis patients in Saudi Arabia. Saudi Med J. 2003;24(Suppl 2):S87–91.

    PubMed  Google Scholar 

  32. Ali A, et al. Determination of HCV genotypes and viral loads in chronic HCV infected patients of Hazara Pakistan. Virol J. 2011;8:466.

    Article  Google Scholar 

  33. Tallo T, Norder H, Tefanova V, Krispin T, Schmidt J, Ilmoja M, et al. Genetic characterization of hepatitis C virus strains in Estonia: fluctuations in the predominating subtype with time. J Med Virol. 2007;79(4):374–82.

    Article  Google Scholar 

  34. Jansons, J., et al., Hepatitis C virus molecular epidemiology in Latvia. Vol. 676. 2004. 65–70.

  35. Ambrozaitis A, Z Agminas KS, Balc Iūnaite G, Widell A. Hepatitis C in Lithuania: incidence, prevalence, risk factors and viral genotypes. Clin Diagn Virol. 1995;4(4):273–84.

    Article  CAS  Google Scholar 

  36. Gasim GI. Comment on: “hepatitis C virus in Arab world: a state of concern”. ScientificWorldJournal. 2012;2012:534173.

    Article  Google Scholar 

  37. Kalinina O, Norder H, Vetrov T, Zhdanov K, Barzunova M, Plotnikova V, et al. Shift in predominating subtype of HCV from 1b to 3a in St. Petersburg mediated by increase in injecting drug use. J Med Virol. 2001;65(3):517–24.

    Article  CAS  Google Scholar 

  38. Ross RS, Viazov S, Renzing-Köhler K, Roggendorf M. Changes in the epidemiology of hepatitis C infection in Germany: shift in the predominance of hepatitis C subtypes. J Med Virol. 2000;60(2):122–5.

    Article  CAS  Google Scholar 

  39. Payan C, Roudot-Thoraval F, Marcellin P, Bled N, Duverlie G, Fouchard-Hubert I, et al. Changing of hepatitis C virus genotype patterns in France at the beginning of the third millenium: the GEMHEP GenoCII study. J Viral Hepat. 2005;12(4):405–13.

    Article  CAS  Google Scholar 

  40. Seme K, Vrhovac M, Mocilnik T, Maticic M, Lesnicar G, Baklan Z, et al. Hepatitis C virus genotypes in 1,504 patients in Slovenia, 1993-2007. J Med Virol. 2009;81(4):634–9.

    Article  Google Scholar 

Download references

Acknowledgments

The authors thank the Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran, for supporting this study and all the volunteers who played a key role in carrying out this research.

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Authors and Affiliations

  1. Department of Biology Ahar Branch, Islamic Azad University, Ahar, Iran

    Saeed Tarzi, Rozita Tamjidifar & Changiz Ahmadizadeh

  2. The University of Tehran, Department of Applied Chemistry, Tehran, Iran

    Mahmoud Mansouri

  3. Amirkabir University of Technology (Polytechnic of Tehran), Department of Applied Chemistry, Tehran, Iran

    Sahar Pashaei Sarand

  4. Department of Immunology, Tabriz University of Medical Sciences, Tabriz, Iran

    Navid Shomali

  5. Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Navid Shomali

Authors
  1. Saeed Tarzi
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  2. Mahmoud Mansouri
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  3. Sahar Pashaei Sarand
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  4. Navid Shomali
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  5. Rozita Tamjidifar
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  6. Changiz Ahmadizadeh
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Correspondence to Changiz Ahmadizadeh.

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Ethics Approval and Consent to Participate

Informed consent was obtained from each patient, and the ethical approaches were approved by the ethics committee of Tabriz University of Medical Sciences.

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The authors declare that they have no conflict of interest.

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Tarzi, S., Mansouri, M., Sarand, S.P. et al. Frequency of Hepatitis C Virus Genotypes in Patients Who Had Hepatocellular Carcinoma in Gastroenterological Hospitals of Tabriz. J Gastrointest Canc 52, 145–149 (2021). https://doi.org/10.1007/s12029-020-00367-2

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  • DOI: https://doi.org/10.1007/s12029-020-00367-2

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