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TEKT4 Promotes Papillary Thyroid Cancer Cell Proliferation, Colony Formation, and Metastasis through Activating PI3K/Akt Pathway

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Abstract

Thyroid carcinoma is the most common malignancy of the endocrine system worldwide, but its molecular mechanisms remain unclear. Some diseases are associated with TEKT4 gene. However, its role in thyroid carcinoma has yet to be fully examined. This study was designed to investigate the function of TEKT4 in papillary thyroid cancer (PTC). The effect of TEKT4 on aggressive behavior of PTC cell lines, namely, TPC1 and BCPAP, transfected with small interfering RNA was identified through cell proliferation, colony formation, migration, and invasion. Our previous study revealed that TEKT4 may be vital in PTC. In in vitro experiments, TEKT4 downregulation suppressed cell proliferation, colony formation, cell migration, and invasion. Our data also indicated that tumor-suppressing role of TEKT4 knockdown in PTC cell lines was associated with the silence of the PI3K/Akt pathway. Our study revealed that TEKT4 shows important biological implications and is worthy of further study.

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Funding

This work was supported by a grant from the Major Science and Technology Projects of Zhejiang Province (2015C03052), Wenzhou Science and Technology Bureau (NO.2017Y0929), and Scientific Research Incubator Project of The First Affiliated Hospital of Wenzhou Medical University (NO. FHY2014018).

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Author notes

  1. Zhouci Zheng and Xiaofen Zhou contributed equally to this work.

Authors and Affiliations

  1. Departments of Neck Surgery, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China

    Zhouci Zheng

  2. Departments of Thyroid and Breast Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China

    Xiaofen Zhou, Yefeng Cai, Endong Chen, Xiaohua Zhang, Ouchen Wang, Qingxuan Wang & Haiguang Liu

Authors
  1. Zhouci Zheng
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  2. Xiaofen Zhou
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  3. Yefeng Cai
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  4. Endong Chen
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  5. Xiaohua Zhang
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  6. Ouchen Wang
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  7. Qingxuan Wang
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  8. Haiguang Liu
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Corresponding authors

Correspondence to Qingxuan Wang or Haiguang Liu.

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The authors declare that they have no conflict of interest.

Ethical Approval

Ethical approval for this study was obtained from the Ethical Committee of the First Affiliated Hospital of Wenzhou Medical University.

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Written informed consent was obtained from each individual participant.

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Zheng, Z., Zhou, X., Cai, Y. et al. TEKT4 Promotes Papillary Thyroid Cancer Cell Proliferation, Colony Formation, and Metastasis through Activating PI3K/Akt Pathway. Endocr Pathol 29, 310–316 (2018). https://doi.org/10.1007/s12022-018-9549-0

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  • DOI: https://doi.org/10.1007/s12022-018-9549-0

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