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Detection and identification of circulating tumor cells in parathyroid tumors and correlation analysis with clinicopathological features

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Abstract

Introduction

The differential diagnosis of parathyroid carcinoma (PC)/parathyroid adenoma (PA) in parathyroid tumors is critical for their management and prognosis. Circulating tumor cells (CTCs) identification in the peripheral blood of parathyroid tumors remains unknown. In this study, we proposed to investigate the differences of CTCs in PC/PA and the relationship with clinicopathologic features to assess its relevance to PC and value in identifying PC/PA.

Methods and materials

Peripheral blood was collected from 27 patients with PC and 37 patients with PA treated in our hospital, and the number of chromosome 8 aberrant CTCs was detected by negative magnetic bead sorting fluorescence in situ hybridization (NE-FISH). The differences of CTCs in PC/PA peripheral blood were compared and their diagnostic efficacy was evaluated, and the correlation between CTCs and clinicopathological features of PC was further explored.

Results

CTCs differed significantly in PC/PA (p = 0.0008) and were up-regulated in PC, with good diagnostic efficacy. CTCs combined with alkaline phosphatase (ALP) assay improved the diagnostic efficacy in identifying PC/PA (AUC = 0.7838, p = 0.0001). The number of CTCs was correlated with tumor dimensions, but not significantly correlated with clinical markers such as calcium and PTH and pathological features such as vascular invasion, lymph node metastasis and distant metastasis.

Conclusion

As a non-invasive liquid biopsy method, CTCs test combined with ALP test can be used as an important reference basis for timely and accurate identification and treatment of PC. It is of great significance to improve the current situation of PC diagnosis, treatment and prognosis.

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Data availability

No datasets were generated or analysed during the current study.

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Acknowledgements

We appreciated Chaoyang district social development program topic for providing funding for this study. The authors would like to express their gratitude to EditSprings for the expert linguistic services provided.

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Authors and Affiliations

  1. Department of Thyroid and Neck Surgery, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, 100020, China

    Jiacheng Wang, Qian Wang, Teng Zhao, Hong Shen, Xing Liu, Dalin Feng, Rongfang Shen, Yuting Wang, Wenjing Yang & Bojun Wei

  2. Department of Pathology, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, 100020, China

    Xingran Jiang

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  1. Jiacheng Wang
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  3. Qian Wang
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  7. Dalin Feng
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Contributions

Jiacheng Wang analyzed and interpreted the experimental data, and was the major contributor in writing the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Bojun Wei.

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The authors declare that they have no conflict of interest.

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This study was approved by the Ethics Review Board of Beijing Chao-Yang Hospital, Capital Medical University.

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All patients have signed a written informed consent to participate in this work.

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CTCs Identification in Parathyroid Tumors

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Wang, J., Jiang, X., Wang, Q. et al. Detection and identification of circulating tumor cells in parathyroid tumors and correlation analysis with clinicopathological features. Endocrine (2024). https://doi.org/10.1007/s12020-024-03831-2

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