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Expression of miR-31-5p affects growth, migration and invasiveness of papillary thyroid cancer cells

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Abstract

Purpose

In this study, we evaluated the biological role of miRNA-31-5p in papillary thyroid cancer (PTC).

Methods

By using the real-time PCR, we measured miRNA-31-5p expression levels in 25 PTC tissues and in two human PTC cell lines (K1 and TPC-1). Then, K1 cells were transiently transfected with mirVana inhibitor or mirVana mimic to miRNA-31-5-p. Cell proliferation was determined by MTT and colony formation assays. The in vitro metastatic ability of thyroid cancer cells was evaluated by adhesion, migration and invasion assays. Epithelial mesenchymal transition (EMT) and Hippo pathway related gene and protein levels were evaluated by using the TaqMan™ Gene Expression Assays and western blot analysis, respectively.

Results

We found a significant increase of miR-31-5-p expression in tumor tissue and in K1 cells harboring the BRAF p.V600E mutation. Knockdown of miR-31-5p determined a reduction of cell proliferation, associated with a significant decrease in cell adhesion, migration and invasion properties. A downregulation of EMT markers and YAP/β-catenin axis was also observed.

Conclusions

Our findings suggest that miRNA-31-5p acts as oncogenic miRNA in human thyrocytes and its overexpression may be involved in the BRAF-related tumorigenesis in PTCs, providing new understanding into its pathological role in PTC progression and invasiveness.

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Acknowledgements

This paper was financially supported by the Department of Health Sciences, University of Catanzaro “Magna Græcia”.

Funding

This study was funded by grants from the Italian Ministry of Universities and Research, PRIN2017EKMFTN_003.

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Author notes

  1. These authors contributed equally: Valentina Maggisano, Francesca Capriglione

Authors and Affiliations

  1. Department of Health Sciences, University “Magna Graecia” of Catanzaro, 88100, Catanzaro, Italy

    Valentina Maggisano, Francesca Capriglione, Marilena Celano, Diego Russo & Stefania Bulotta

  2. Department of Translational and Precision Medicine, “Sapienza” University of Rome, 00161, Rome, Italy

    Antonella Verrienti, Marialuisa Sponziello, Valeria Pecce & Cosimo Durante

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  1. Valentina Maggisano
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Contributions

Conceptualization, V.M., F.C., and D.R.; Investigation, V.M., F.C., A.V., and M.C.; Data curation, A.V., M.S., V.P., M.C.; Writing-original draft preparation, V.M., S.B., and D.R.; Writing-review and editing, C.D., S.B., and D.R.; Supervision, C.D., D.R., and S.B. All authors have read and agreed to the published version of the paper.

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Correspondence to Stefania Bulotta.

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This study was conducted according to guidelines of the Declaration of Helsinki and approved by the ethics committee of Sapienza University of Rome (protocol code 1184/17 and date of approval 21/12/2017).

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Maggisano, V., Capriglione, F., Verrienti, A. et al. Expression of miR-31-5p affects growth, migration and invasiveness of papillary thyroid cancer cells. Endocrine 79, 517–526 (2023). https://doi.org/10.1007/s12020-022-03267-6

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