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Expression and localization of the sodium/iodide symporter (NIS) in testicular cells

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Abstract

Administration of radioiodine (I131) is currently exploited for both diagnostic and therapeutic treatment of thyroid cancer. Few data are available on the sodium/iodide symporter (NIS) expression in human testis, a particular important prerequisite to predict radioiodine accumulation in the gonads of males with thyroid cancer exposed to such a treatment. In this study, we analyzed the expression of NIS in mouse, rat and human normal testis in different stages of development. By using a quantitative RT-PCR, Western blot and immunohistochemical analysis, NIS mRNA and protein were measured in both fetal and adult testicular tissues. NIS transcript was detected in both fetal and adult testis, although its expression levels were approximately 10-fold less than in thyroid gland. Western blot analysis and immunohistochemistry showed the presence of NIS protein in germinal and Leydig cells, but not in Sertoli cells with prevalent expression in the cytosol compartment of the cells. Our study demonstrates that NIS transcript and protein are expressed in normal testis. Further studies will demonstrate whether it may act as the transporter of radioiodine in normal testis of male patients with thyroid cancer.

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Acknowledgments

This work was supported by a grant from MIUR (PRIN-Cofin 2008) to D.R. V. Maggisano is fellow of PhD in Pharmaceutical Sciences at University of Catanzaro.

Conflict of interest

The authors declare that the experiments comply with the current laws of the country in which they were performed and that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Pharmacobiological Sciences, University of Catanzaro “Magna Graecia”, Viale Europa, 88100, Germaneto, Catanzaro, Italy

    Diego Russo & Valentina Maggisano

  2. Department of Internal Medicine and Medical Specialties, University of Roma “Sapienza”, V.le del Policlinico, 155-00161, Rome, Italy

    Angela Scipioni, Cosimo Durante, Antonella Verrienti & Sebastiano Filetti

  3. Department of Experimental Medicine, University of Roma “Sapienza”, 00161, Rome, Italy

    Elisabetta Ferretti

  4. Department of Medical Pathophysiology, University of Roma “Sapienza”, 00161, Rome, Italy

    Loredana Gandini, Donatella Paoli & Andrea Lenzi

  5. Department of Clinical and Experimental Medicine, University of Catanzaro “Magna Graecia”, Viale Europa, 88100, Germaneto, Catanzaro, Italy

    Giuseppe Costante

Authors
  1. Diego Russo
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  2. Angela Scipioni
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  3. Cosimo Durante
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  4. Elisabetta Ferretti
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  5. Loredana Gandini
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  6. Valentina Maggisano
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  9. Giuseppe Costante
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  10. Andrea Lenzi
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  11. Sebastiano Filetti
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Correspondence to Diego Russo or Sebastiano Filetti.

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Russo, D., Scipioni, A., Durante, C. et al. Expression and localization of the sodium/iodide symporter (NIS) in testicular cells. Endocrine 40, 35–40 (2011). https://doi.org/10.1007/s12020-011-9469-y

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  • DOI: https://doi.org/10.1007/s12020-011-9469-y

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