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Expression of Iodotyrosine Deiodinase in Thyroid and Other Organs in Iodine-Deficient and Iodine-Excess Rats

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Abstract

In mammals, iodothyronine deiodinase and iodotyrosine deiodinase (IYD) are known to catalyze the reductive dehalogenation. IYD is a critical enzyme in maintaining iodine homeostasis. Advances in the study of iodothyronine deiodinase have been published steadily; research on IYD has been slow on its function and regulation. We studied the expression of IYD in thyroid, liver, and kidney in conditions such as iodine deficiency and excess to determine its regulation and role in iodine recycling. Sixty 4-week-old female Wistar rats were randomly divided into two groups, with each group containing three subgroups. The rats were fed with different iodine intake for 3 months. After 3 months, all the rats were sacrificed, and the expression of IYD in thyroid, liver, and kidney of the rats were determined. We found that the expression of thyroidal IYD in 0.3-fold-iodine intake group was significantly higher as compared with the low-iodine feed control group (p < 0.01), whereas the expression in 6-fold-iodine intake group was significantly decreased as compared with normal-iodine feed control group (p < 0.01). However, the variation of IYD expression in thyroid was not similar to liver and kidney. In conclusion, iodine deficiency results in an increased expression of IYD in thyroid, whereas excess iodine decreases the expression of thyroidal IYD. In humans, daily iodine intake of <75 or >500 μg can affect the expression of thyroidal IYD. The safety range of iodine intake is narrow. In addition, further investigations are required to study the expression and regulation of IYD in various organs.

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References

  1. Delange F (2001) Iodine deficiency as a cause of brain damage. Postgrad Med J 77:217–220

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  2. Zimmermann MB (2011) The role of iodine in human growth and development. Semin Cell Dev Biol 22:645–652

    Article  CAS  PubMed  Google Scholar 

  3. Zimmermann MB (2009) Iodine deficiency. Endocr Rev 30:376–408

    Article  CAS  PubMed  Google Scholar 

  4. Kopp PA (2008) Reduce, recycle, reuse—iodotyrosine deiodinase in thyroid iodide metabolism. N Engl J Med 358:1856–1859

    Article  CAS  PubMed  Google Scholar 

  5. Roche J, Michel R, Michel O, Lissitzky S (1952) Sur la déshalogénation enzymatique des iodotyrosines par le corps thyroïde et sur son rôle physiologique. Biochim Biophys Acta 9:161–169

    Article  CAS  PubMed  Google Scholar 

  6. Rosenberg IN, Goswami A (1979) Purification and characterization of a flavoprotein from bovine thyroid with iodotyrosine deiodinase activity. J Biol Chem 254:12318–12325

    CAS  PubMed  Google Scholar 

  7. Moreno JC, Pauws E, van Kampen AH, Jedlickova M, de Vijlder JJ, Ris-Stalpers C (2001) Cloning of tissue-specific genes using serial analysis of gene expression and a novel computational substraction approach. Genomics 75:70–76

    Article  CAS  PubMed  Google Scholar 

  8. Moreno JC (2003) Identification of novel genes involved in congenital hypothyroidism using serial analysis of gene expression. Horm Res 60(Suppl 3):96–102

    Article  CAS  PubMed  Google Scholar 

  9. Gnidehou S, Lacroix L, Sezan A, Ohayon R, Noel-Hudson MS, Morand S, Francon J, Courtin F, Virion A, Dupuy C (2006) Cloning and characterization of a novel isoform of iodotyrosine dehalogenase 1 (DEHAL1) DEHAL1C from human thyroid: comparisons with DEHAL1 and DEHAL1B. Thyroid 16:715–724

    Article  CAS  PubMed  Google Scholar 

  10. Maayan ML, Rosenberg IN (1963) Effect of injection of thyrotropin upon deiodination of diiodotyrosine by rat thyroid and liver. Endocrinology 73:38–44

    Article  CAS  PubMed  Google Scholar 

  11. Gnidehou S, Caillou B, Talbot M, Ohayon R, Kaniewski J, Noel-Hudson MS, Morand S, Agnangji D, Sezan A, Courtin F, Virion A, Dupuy C (2004) Iodotyrosine dehalogenase 1 (DEHAL1) is a transmembrane protein involved in the recycling of iodide close to the thyroglobulin iodination site. FASEB J 18:1574–1576

    CAS  PubMed  Google Scholar 

  12. Moreno JC, Klootwijk W, van Toor H, Pinto G, D'Alessandro M, Leger A, Goudie D, Polak M, Gruters A, Visser TJ (2008) Mutations in the iodotyrosine deiodinase gene and hypothyroidism. N Engl J Med 358:1811–1818

    Article  CAS  PubMed  Google Scholar 

  13. Macours P, Aubry JC, Hauquier B, Boeynaems JM, Goldman S, Moreno-Reyes R (2008) Determination of urinary iodine by inductively coupled plasma mass spectrometry. J Trace Elem Med Biol 22:162–165

    Article  CAS  PubMed  Google Scholar 

  14. Rokita SE, Adler JM, McTamney PM, Watson JJ (2010) Efficient use and recycling of the micronutrient iodide in mammals. Biochimie 92:1227–1235

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  15. Solis-S JC, Villalobos P, Orozco A, Delgado G, Quintanar-Stephano A, Garcia-Solis P, Hernandez-Montiel HL, Robles-Osorio L, Valverde-R C (2011) Inhibition of intrathyroidal dehalogenation by iodide. J Endocrinol 208:89–96

    Article  CAS  PubMed  Google Scholar 

  16. Shimizu R, Yamaguchi M, Uramaru N, Kuroki H, Ohta S, Kitamura S, Sugihara K (2013) Structure–activity relationships of 44 halogenated compounds for iodotyrosine deiodinase-inhibitory activity. Toxicology 314:22–29

    Article  CAS  PubMed  Google Scholar 

  17. Delange F, Burgi H, Chen ZP, Dunn JT (2002) World status of monitoring iodine deficiency disorders control programs. Thyroid 12:915–924

    Article  PubMed  Google Scholar 

  18. WHO, UNICEF, ICCIDD (2001) Assessment of iodine deficiency disorders and monitoring their elimination. Geneva, World Health Organization (WHO/NHD/01.1)

  19. de Benoist B, McLean E, Andersson M, Rogers L (2008) Iodine deficiency in 2007: global progress since 2003. Food Nutr Bull 29:195–202

    Article  PubMed  Google Scholar 

  20. Teng W, Shan Z, Teng X, Guan H, Li Y, Teng D, Jin Y, Yu X, Fan C, Chong W, Yang F, Dai H, Yu Y, Li J, Chen Y, Zhao D, Shi X, Hu F, Mao J, Gu X, Yang R, Tong Y, Wang W, Gao T, Li C (2006) Effect of iodine intake on thyroid diseases in China. N Engl J Med 354:2783–2793

    Article  CAS  PubMed  Google Scholar 

  21. Trumbo P, Yates AA, Schlicker S, Poos M (2001) Dietary reference intakes: vitamin A, vitamin K, arsenic, boron, chromium, copper, iodine, iron, manganese, molybdenum, nickel, silicon, vanadium, and zinc. J Am Diet Assoc 101:294–301

    Article  CAS  PubMed  Google Scholar 

  22. European Commission HCPD-GSCF (2002) Opinion of the Scientific Committee on Food on the tolerable upper level of intake of iodine. European Commission, Brussels

    Google Scholar 

  23. Kohrle J, Jakob F, Contempre B, Dumont JE (2005) Selenium, the thyroid, and the endocrine system. Endocr Rev 26:944–984

    Article  CAS  PubMed  Google Scholar 

  24. Lavado-Autric R, Calvo RM, de Mena RM, de Escobar GM, Obregon MJ (2013) Deiodinase activities in thyroids and tissues of iodine-deficient female rats. Endocrinology 154:529–536

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Endocrinology and Metabolism, Institute of Endocrinology, Liaoning Provincial Key Laboratory of Endocrine Diseases, The First Affiliated Hospital of China Medical University, No.155 Nanjing North Street, Shenyang, 110001, People’s Republic of China

    Xin Sun, Xiaowen Zhang, Yaqiu Jiang, Suqing Bao, Zhongyan Shan & Weiping Teng

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  1. Xin Sun
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  2. Xiaowen Zhang
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  3. Yaqiu Jiang
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  4. Suqing Bao
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  5. Zhongyan Shan
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  6. Weiping Teng
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Correspondence to Weiping Teng.

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Xin Sun and Xiaowen Zhang contributed equally to this work.

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Sun, X., Zhang, X., Jiang, Y. et al. Expression of Iodotyrosine Deiodinase in Thyroid and Other Organs in Iodine-Deficient and Iodine-Excess Rats. Biol Trace Elem Res 167, 272–279 (2015). https://doi.org/10.1007/s12011-015-0328-1

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  • DOI: https://doi.org/10.1007/s12011-015-0328-1

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