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MicroRNA Expression Signatures and Their Correlation with Clinicopathological Features in Glioblastoma Multiforme

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Abstract

The increasing interest in identifying molecular biomarkers to determine patient prognosis in glioblastoma multiforme (GBM) has resulted in several microRNA (miRNA)-based signatures able to predict progression-free and overall survival. However, the coherency between these signatures is small, and correlations to clinicopathological features other than survival are seldom seen. The aim of this study was to identify any significant relationship between miRNA signatures and clinicopathological data by combining pathological features with miRNA and mRNA analysis in fourteen GBM patients. In total, 161 miRNAs were shown to cluster the GBM tumor samples into long- and short-term-surviving patients. Many of these miRNAs were associated with differential expression in GBM, including a number of miRNAs shown to confer risk or protection with respect to clinical outcome and to modulate the mesenchymal mode of migration and invasion. An inverse relationship between miR-125b and nestin expression was identified and correlated with overall survival in GBM patients, eloquently illustrating how clinicopathological findings and molecular profiling may be a relevant combination to predict patient outcome. The intriguing finding that many of the differentially expressed miRNAs contained exosome-packaging motifs in their mature sequences suggests that we must expand our view to encompass the complex intercellular communication in order to identify molecular prognostic biomarkers and to increase our knowledge in the field of GBM pathogenesis.

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Abbreviations

3′UTR:

3′ Untranslated region

5ALA:

5-Aminolevulinic acid

CNS:

Central nervous system

EGFR:

Epidermal growth factor receptor

EXO-motif:

Exosome-packaging-associated motif

GBM:

Glioblastoma multiforme

GFAP:

Glial fibrillary acidic protein

IDH1:

Isocitrate dehydrogenase 1

MAD:

Median absolute deviation

MGMT:

Methyl guanine methyl transferase

miRNA:

MicroRNA

MMMI:

Mesenchymal mode of migration and invasion

mRNA:

Messenger RNA

RNA:

Ribonucleic acid

TCGA:

The Cancer Genome Atlas

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Acknowledgments

The authors would like to acknowledge laboratory technician Rikke Sophie Holm Kristensen, Aalborg University for her excellent technical assistance. Sille Jensen, Julie Vedel, Spogmai Zadran, Ditte Hansen and Line Tegtmeier Frandsen are acknowledged for their help in gathering the clinical data. This work was supported by grants from Det Obelske Familiefond and Spar Nord Fonden. Kasper Bendix Johnsen is supported by the Novo Scholarship Programme (Novo Nordisk, Denmark).

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Authors and Affiliations

  1. Laboratory for Cancer Biology, Institute of Health Science and Technology, Aalborg University, Fredrik Bajers Vej 3B, 9220, Aalborg Ø, Denmark

    Michael Henriksen, Kasper Bendix Johnsen, Pia Olesen, Linda Pilgaard & Meg Duroux

  2. Laboratory for Neurobiology, Institute of Health Science and Technology, Aalborg University, Fredrik Bajers Vej 3B, 9220, Aalborg Ø, Denmark

    Kasper Bendix Johnsen

  3. Department of Neurosurgery, Aalborg University Hospital, Hobrovej 18-22, Postbox 365, 9100, Aalborg, Denmark

    Pia Olesen

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  1. Michael Henriksen
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Correspondence to Meg Duroux.

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Henriksen, M., Johnsen, K.B., Olesen, P. et al. MicroRNA Expression Signatures and Their Correlation with Clinicopathological Features in Glioblastoma Multiforme. Neuromol Med 16, 565–577 (2014). https://doi.org/10.1007/s12017-014-8309-7

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