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2-Hexadecenal Regulates ROS Production and Induces Apoptosis in Polymorphonuclear Leucocytes

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Abstract

2-Hexadecenal (2-HD)—a biologically active long-chain fatty aldehyde formed in organism enzymatically or nonenzymatically in the reaction of free-radical destruction of sphingolipids under the action of hypochlorous acid, producing by myeloperoxidase. This research aimed to study 2-HD effects on polymorphonuclear leukocytes’ (PMNLs) functions. It has been shown that at submicromolar concentrations, 2-HD causes an elevation in ROS production by PMNLs. It has been found that such effect is associated with signal transduction pathways modification and expressed in elevation of NADPH oxidase, MPO, and JNK-MAPK contributions to this process. At higher concentrations, 2-HD induces apoptosis, which correlates with a significant increase in free Ca2+ in the cytoplasm, a decrease in ROS production, and a decline in mitochondrial potential. Both of these processes are accompanied by cytoskeleton reorganization.

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Data availability

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

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Funding

This study was funded by the Ministry of Education of Belarus [grant number 20180589] and the World Federation of Scientists.

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Author notes

  1. Alexandra G. Lisovskaya

    Present address: Notre Dame Radiation Laboratory, University of Notre Dame, Notre Dame, IN, 46556, USA

Authors and Affiliations

  1. Department of Radiation Chemistry and Chemical-Pharmaceutical Technologies, Faculty of Chemistry, Belarusian State University, 4, Nezavisimosti av, 220030, Minsk, Belarus

    Galina N. Semenkova, Nadezda V. Amaegberi, Alexandra G. Lisovskaya & Oleg I. Shadyro

  2. Institute of Biophysics and Cell Engineering of NAS of Belarus, 27, Academicheskaya st, 220072, Minsk, Belarus

    Serge V. Pinchuk & Аnna G. Poleshko

  3. Research Institute for Physical and Chemical Problems, 14 Leningradskaya st., 220030, Minsk, Belarus

    Oleg I. Shadyro

Authors
  1. Galina N. Semenkova
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  2. Nadezda V. Amaegberi
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  3. Alexandra G. Lisovskaya
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  4. Serge V. Pinchuk
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  6. Oleg I. Shadyro
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Contributions

Galina Semenkova and Oleg Shadyro conceived and designed the experimental study. Nadezda Amaegberi, Alexandra Lisovskaya, Anna Poleshko and Serge Pinchuk performed the experiments, acquired and analyze data. All authors participated in the writing of manuscript. All authors read and approved the final version of manuscript.

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Correspondence to Nadezda V. Amaegberi.

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Semenkova, G.N., Amaegberi, N.V., Lisovskaya, A.G. et al. 2-Hexadecenal Regulates ROS Production and Induces Apoptosis in Polymorphonuclear Leucocytes. Cell Biochem Biophys 81, 77–86 (2023). https://doi.org/10.1007/s12013-022-01117-w

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