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Concurrent Longitudinal EPR Monitoring of Tissue Oxygenation, Acidosis, and Reducing Capacity in Mouse Xenograft Tumor Models

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Abstract

Tissue oxygenation, extracellular acidity, and tissue reducing capacity are among crucial parameters of tumor microenvironment (TME) of significant importance for tumor pathophysiology. In this paper, we demonstrate the complementary application of particulate lithium octa-n-butoxy-naphthalocyanine and soluble nitroxide paramagnetic probes for monitoring of these TME parameters using electron paramagnetic resonance (EPR) technique. Two different types of therapeutic interventions were studied: hypothermia and systemic administration of metabolically active drug. In summary, the results demonstrate the utility of EPR technique for non-invasive concurrent longitudinal monitoring of physiologically relevant chemical parameters of TME in mouse xenograft tumor models, including that under therapeutic intervention.

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Acknowledgments

This work was partially supported by NIH grants CA194013, CA192064, and U54GM104942.

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Authors and Affiliations

  1. In Vivo Multifunctional Magnetic Resonance Center, Robert C. Byrd Health Science Center, West Virginia University, Morgantown, WV, 26506, USA

    Andrey A. Bobko & Valery V. Khramtsov

  2. Department of Biochemistry, West Virginia University School of Medicine, Morgantown, WV, 26506, USA

    Andrey A. Bobko & Valery V. Khramtsov

  3. The James Comprehensive Cancer Center, The Ohio State University, Columbus, OH, USA

    Jason Evans & Nicholas C. Denko

Authors
  1. Andrey A. Bobko
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  2. Jason Evans
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  3. Nicholas C. Denko
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  4. Valery V. Khramtsov
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Correspondence to Valery V. Khramtsov.

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Bobko, A.A., Evans, J., Denko, N.C. et al. Concurrent Longitudinal EPR Monitoring of Tissue Oxygenation, Acidosis, and Reducing Capacity in Mouse Xenograft Tumor Models. Cell Biochem Biophys 75, 247–253 (2017). https://doi.org/10.1007/s12013-016-0733-x

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  • DOI: https://doi.org/10.1007/s12013-016-0733-x

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